Association of polymorphisms of ABCA1 and ROS1 with hypertension in Japanese individuals.
Association of polymorphisms of ABCA1 and ROS1 with hypertension in Japanese individuals.
复制标题
DOI：
10.3892/ijmm.21.1.83
复制
发表时间：
2008
影响因子：
5.4
通讯作者：
Yoshiji Yamada;Kimihiko Kato;Tetsuro Yoshida;K. Yokoi;H. Matsuo;Sachiro Watanabe;S. Ichihara;N. Metoki;H. Yoshida;K. Satoh;Y. Aoyagi;A. Yasunaga;Hyuntae Park;Masashi Tanaka;Y. Nozawa
中科院分区：
文献类型：
--
作者：
Yoshiji Yamada;Kimihiko Kato;Tetsuro Yoshida;K. Yokoi;H. Matsuo;Sachiro Watanabe;S. Ichihara;N. Metoki;H. Yoshida;K. Satoh;Y. Aoyagi;A. Yasunaga;Hyuntae Park;Masashi Tanaka;Y. Nozawa
虽然各种环境因素，如高盐饮食，吸烟习惯，过量饮酒和身体活动不足，影响高血压的发展，遗传变异也有助于个体对这种情况的易感性。本研究的目的是确定基因多态性，赋予易感性或抵抗高血压，从而有助于预测这种情况的遗传风险。研究人群包括2752名无关的日本个体（1370名男性，1382名女性），包括1276名高血压受试者（774名男性，502名女性）和1476名对照（596名男性，880名女性）。采用聚合酶链反应和序列特异性寡核苷酸探针结合悬浮芯片技术对35个候选基因的50个多态性位点进行基因型检测。通过卡方检验和随后的多变量logistic回归分析对基因型分布进行评估，并调整年龄、性别、体重指数、吸烟状况以及糖尿病和高胆固醇血症的患病率，结果显示ABCA 1的-14 C-->T多态性，C-->G多态性，（Ser 2229 Cys）多态性，C->T低密度脂蛋白受体（LDLR）的Asn 591 Asn多态性，13989 A-->G CYP 3A 4的Ile 118 Val多态性，ADIPOR 1的C->G和A->C多态性，MMP 1基因-519A-->G多态性（ABCA 1的PT多态性和ROS 1的C-->G（Ser 2229 Cys）多态性）分别与血压升高相关，其中T和G等位基因的变异与血压升高相关。这些结果表明，ABCA 1和ROS 1的多态性是日本人血压和高血压发展的决定因素。因此，ABCA 1和ROS 1基因型的测定可能为高血压遗传风险的预测提供信息。
Although various environmental factors, such as a high-salt diet, a smoking habit, excessive alcohol intake, and physical inactivity, influence the development of hypertension, genetic variation also contributes to an individual's susceptibility to this condition. The purpose of the present study was to identify gene polymorphisms that confer susceptibility or resistance to hypertension, and thereby contribute to the prediction of the genetic risk for this condition. The study population comprised 2752 unrelated Japanese individuals (1370 men, 1382 women), including 1276 subjects with hypertension (774 men, 502 women) and 1476 controls (596 men, 880 women). The genotypes for 50 polymorphisms of 35 candidate genes were determined by a method that combines polymerase chain reaction and sequence-specific oligonucleotide probes with suspension array technology. Evaluation of genotype distributions by the Chi-square test and subsequent multivariable logistic regression analysis with adjustment for age, sex, body mass index, smoking status, and the prevalence of diabetes mellitus and hypercholesterolemia revealed that the -14C-->T polymorphism of ABCA1, the C-->G (Ser2229Cys) polymorphism of ROS1, the C-->T (Asn591Asn) polymorphism of LDLR, the 13989A-->G (Ile118Val) polymorphism of CYP3A4, the C-->G and A-->C polymorphisms of ADIPOR1, and the -519A-->G polymorphism of MMP1 were significantly (PT polymorphism of ABCA1 and the C-->G (Ser2229Cys) polymorphism of ROS1, with the variant T and G alleles, respectively, being related to increased blood pressure. These results suggest that polymorphisms of ABCA1 and ROS1 are determinants of blood pressure and the development of hypertension in Japanese individuals. Determination of genotypes for ABCA1 and ROS1 may thus prove informative for the prediction of the genetic risk for hypertension.