Human IgE and IgG antibodies to mosquito proteins detected by the immunoblot technique.
Human IgE and IgG antibodies to mosquito proteins detected by the immunoblot technique.
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通过免疫印迹技术检测到针对蚊子蛋白的人类 IgE 和 IgG 抗体。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
S. H. Han
中科院分区：
文献类型：
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作者：
H. Shen;C. C. Chen;H. N. Chang;L. Y. Chang;W. C. Tu;S. H. Han
本文应用免疫印迹技术检测了人血清中抗蚊抗原的IgE和IgG抗体。在SDS-聚丙烯酰胺凝胶上分离苔藓蛋白，并将其电泳转移到硝酸纤维素纸上。在与来自不同个体的血清孵育后，用酶标记的山羊抗人免疫球蛋白分析沉淀的条带。IgE和IgG抗体识别不同的抗原模式。
Immunoblot technique was used in this study to detect IgE and IgG antibodies in human sera against mosquito antigens. Mosquito proteins were separated on SDS-polyacrylamide gels and transferred electrophoretically to nitrocellulose papers. After incubation with sera from different individuals, the precipitated bands were analyzed with enzyme-labeled goat anti-human immunoglobulins. Distinct patterns of antigen were recognized by IgE and IgG antibodies.