The effect of age on the corneal subbasal nerve plexus
The effect of age on the corneal subbasal nerve plexus
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DOI：
10.1097/01.ico.0000154387.51355.39
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发表时间：
2005-08-01
期刊：
影响因子：
2.8
通讯作者：
Bourne, WM
中科院分区：
文献类型：
--
作者：
Erie, JC;McLaren, JW;Bourne, WM
目的：测量不同年龄段正常人角膜基底神经的密度和方位。方法：采用串联扫描共聚焦显微镜对65例正常人的65只角膜进行检查。受试者年龄15~79岁，平均46~19岁，每侧5例。在中央角膜的共聚焦图像中，基底下神经纤维束表现为明亮、清晰的线状结构。每只眼睛3到8次扫描(平均4.6+/-1.8次)的图像被随机呈现给蒙面观察者进行分析。使用定制的软件程序确定平均基础下神经密度(共焦图像内的总神经长度[mum][面积=0.166 mm(2)])、每次共焦扫描的平均神经数和平均神经方位。用Pearson相关系数分析年龄与神经密度、年龄与神经方向的相关性。结果：所有受试者的角膜中央均可见底下神经丛。基础下神经平均密度为8404±2012µm/mm(2例)，范围为4735~14,018µm/mm(2例)。基底神经数目平均为4.6+/-1.6条(1~8条)。基础下神经方向平均94°+/-16°(58°~146°)。年龄与基底神经密度无相关性(r=0.21，P=0.09)，与基底神经方向无相关性(r=-0.19，P=0.12)。结论：人角膜中央基底神经丛的密度和方向不随年龄变化。
Purpose: To measure subbasal nerve density and orientation in normal human corneas across a broad age range.Methods: Sixty-five normal corneas of 65 subjects were examined by using tandem scanning confocal microscopy. Ages of subjects ranged from 15 to 79 years (mean 46 19 years), with 5 subjects from each hemidecade. Subbasal nerve fiber bundles appeared as bright, well-defined linear structures in confocal images of the central cornea. Images from 3 to 8 scans per eye (mean 4.6 +/- 1.8 scans) were randomly presented to a masked observer for analysis. The mean subbasal nerve density (total nerve length [mu m] within a confocal image [area = 0.166 mm(2)]), the mean nerve number per confocal scan, and the mean nerve orientation were determined by using a custom software program. Correlations between age and nerve density and age and nerve orientation were assessed by using Pearson correlation coefficients.Results: The subbasal nerve plexus was visible in the central cornea of all subjects. The mean subbasal nerve density was 8404 +/- 2012 mu m/mm(2) (range 4735 to 14,018 mu m/mm(2)). The mean subbasal nerve number was 4.6 +/- 1.6 nerves (range 1 o 8 nerves). The mean subbasal nerve orientation was 94 +/- 16 degrees (range 58 to 146 degrees). There was no correlation between age and subbasal nerve density (r = 0.21, P = 0.09) or between age and subbasal nerve orientation (r = -0.19, P = 0.12).Conclusion: The density and orientation of the subbasal nerve plexus in the central human cornea does not change with age.