Comparison of physiological and behavioral responses to chronic restraint stress between C57BL/6J and BALB/c mice
Comparison of physiological and behavioral responses to chronic restraint stress between C57BL/6J and BALB/c mice
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C57BL/6J和BALB/c小鼠对慢性束缚应激的生理和行为反应比较
DOI：
10.1016/j.bbrc.2020.02.073
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发表时间：
2020
影响因子：
3.1
通讯作者：
Sohya Kazuhiro
中科院分区：
文献类型：
--
作者：
Tsuchimine Shoko;Matsuno Hitomi;O’Hashi Kazunori;Chiba Shuichi;Yoshimura Aya;Kunugi Hiroshi;Sohya Kazuhiro
啮齿类动物慢性束缚应激（CRS）模型通常被用作抑郁症的简单模型。然而，这些应激模型主要是在大鼠中开发的，CRS模型的行为表型仍然存在争议。在这项研究中，我们比较了C57 BL/6 J（B6）和BALB/c小鼠，这是常用的遗传和行为研究，CRS的生理和行为反应。除了测量生理参数和皮质酮（一种应激激素）水平对应激的反应外，我们还研究了睾酮（一种抗应激激素）水平的变化，这在应激小鼠中很少被研究。将小鼠暴露于CRS，每天6小时，持续21天。在B6和BALB/c小鼠中，CRS引起了几种生理应激反应，包括体重增加减少和应激相关器官的组织重量变化。测量毛发中累积的皮质酮，并且BALB/c小鼠在CRS后具有显著高于对照小鼠和B6小鼠的水平。另一方面，在毛发中积累睾酮的情况下，B6小鼠和BALB/c小鼠均显示出显著高于对照小鼠的浓度，但两种品系之间的变化程度没有差异。在蔗糖偏好实验中，BALB/c小鼠，而不是B6小鼠，在CRS后表现出快感缺失样行为。然而，在强迫游泳或悬尾试验中，两种品系都没有表现出抑郁样行为。我们的研究结果表明，BALB/c小鼠的生理和行为应激反应大于B6小鼠，虽然抗应激反应CRS是相似的两个品系。这表明BALB/c小鼠可能有利于用作CRS诱导的抑郁模型。
Rodent models of chronic restraint stress (CRS) are often used as simple models of depressive disorder. However, these models of stress have been mainly developed in rats, and the behavioral phenotypes of CRS models are still controversial. In this study, we compared the physiological and behavioral responses of C57BL/6J (B6) and BALB/c mice, which are commonly used in genetic and behavioral studies, to CRS. In addition to measuring physiological parameters and the levels of corticosterone (a stress hormone) in response to stress, we also examined changes in the levels of testosterone (an anti-stress hormone), which have rarely been studied in stressed mice. The mice were exposed to CRS for 6 h a day for 21 days. In both B6 and BALB/c mice, CRS elicited several physiological stress responses, including decreased body weight gain and changes in the tissue weights of stress-related organs. Accumulated corticosterone in the hair was measured, and BALB/c mice had significantly greater levels than control mice and B6 mice after CRS. On the other hand, in the case of accumulated testosterone in the hair, both B6 mice and BALB/c mice showed significantly higher concentrations than control mice, but the degree of change was not different between the two strains. In the sucrose preference test, BALB/c mice, but not B6 mice, showed anhedonia-like behavior after CRS. However, neither strain showed depressive-like behavior in the forced swim or tail suspension test. Our results show that the physiological and behavioral stress responses of BALB/c mice are greater than those of B6 mice, although anti-stress responses to CRS are similar in both strains. This suggests that BALB/c mice are likely to be advantageous for use as a CRS-induced depression model.