DISTINCTIVE RESPONSES OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE AND CARBONIC-ANHYDRASE IN WHEAT LEAVES TO NITROGEN NUTRITION AND THEIR POSSIBLE RELATIONSHIPS TO CO2-TRANSFER RESISTANCE
DISTINCTIVE RESPONSES OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE AND CARBONIC-ANHYDRASE IN WHEAT LEAVES TO NITROGEN NUTRITION AND THEIR POSSIBLE RELATIONSHIPS TO CO2-TRANSFER RESISTANCE
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DOI：
10.1104/pp.100.4.1737
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发表时间：
1992-12-01
期刊：
影响因子：
7.4
通讯作者：
OSMOND, B
中科院分区：
文献类型：
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作者：
MAKINO, A;SAKASHITA, H;OSMOND, B
测定了小麦（Triticum aestivum L.）、水稻（Oryza sativa L.）、菠菜（Spinacia oleracea L.）、豆类（Phaseolus vulgaris L.）和豌豆（Pisum sativum L.）完全膨大、幼嫩叶片的核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）、总叶绿素（Chl）和叶片总氮含量。测定了全链电子传递和碳酸酐酶的活性。所有植株均在不同氮浓度下水培。虽然水稻、菠菜、豆类和豌豆的Rubisco含量相对于叶片氮含量和Chl的增加大于成比例，但小麦Rubisco与叶片总氮或Chl的比例基本上与氮处理无关。此外，Rubisco与电子传递活性的比值仅在小麦中保持不变。然而，气体交换分析表明，在小麦、水稻和菠菜中，Rubisco和电子传递能力之间的体内平衡几乎保持不变，与氮处理无关。小麦体外碳酸酐酶活性极低，对氮素含量的增加反应强烈。这种反应在其他C3植物中没有发现，在任何叶片含氮量下，它们的碳酸酐酶活性都比小麦高10到30倍。讨论了小麦碳酸酐酶活性的这些不同反应与抗co2转移能力以及Rubisco和电子传递能力之间的体内平衡的关系。
The amounts of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), total chlorophyll (Chl), and total leaf nitrogen were measured in fully expanded, young leaves of wheat (Triticum aestivum L.), rice (Oryza sativa L.), spinach (Spinacia oleracea L.), bean (Phaseolus vulgaris L), and pea (Pisum sativum L.). In addition, the activities of whole-chain electron transport and carbonic anhydrase were measured. All plants were grown hydroponically at different nitrogen concentrations. Although a greater than proportional increase in Rubisco content relative to leaf nitrogen content and Chl was found with increasing nitrogen supply for rice, spinach, bean, and pea, the ratio of Rubisco to total leaf nitrogen or Chl in wheat was essentially independent of nitrogen treatment. In addition, the ratio of Rubisco to electron transport activities remained constant only in wheat. Nevertheless, gas-exchange analysis showed that the in vivo balance between the capacities of Rubisco and electron transport in wheat, rice, and spinach remained almost constant, irrespective of nitrogen treatment. The in vitro carbonic anhydrase activity in wheat was very low and strongly responsive to increasing nitrogen content. Such a response was not found for the other C3 plants examined, which had 10- to 30-fold higher carbonic anhydrase activity than wheat at any leaf-nitrogen content. These distinctive responses of carbonic anhydrase activity in wheat were discussed in relation to CO2-transfer resistance and the in vivo balance between the capacities of Rubisco and electron transport.