Enhanced tolerance and remediation to mixed contaminates of PCBs and 2,4-DCP by transgenic alfalfa plants expressing the 2,3-dihydroxybiphenyl-1,2-dioxygenase.
Enhanced tolerance and remediation to mixed contaminates of PCBs and 2,4-DCP by transgenic alfalfa plants expressing the 2,3-dihydroxybiphenyl-1,2-dioxygenase.
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DOI：
10.1016/j.jhazmat.2014.12.049
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发表时间：
2015-04
影响因子：
13.6
通讯作者：
Yan Wang;Hejun Ren;H. Pan;Jinliang Liu;Lan-ying Zhang
中科院分区：
文献类型：
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作者：
Yan Wang;Hejun Ren;H. Pan;Jinliang Liu;Lan-ying Zhang
由于商业和农业活动，多氯联苯和2，4-二氯苯酚通常导致土壤混合污染。它们在环境中的积累对人类和动物的健康构成巨大风险。因此，迫切需要有效的处置这些污染物的策略。本研究以基因工程强化PCBs/2，4-DCP植物修复为重点。从土壤宏基因组文库中克隆了芳香族化合物有氧催化转化的关键酶2，3-二羟基联苯-1，2-双加氧酶（BphC.B），并利用农杆菌介导的转化方法，在CaMV 35 S启动子的驱动下，在苜蓿中表达。通过PCR、Western印迹分析和酶活性测定，筛选出转基因系BB 11。研究了其对PCBs和2，4-DCP的处理和耐受性。转基因株系BB 11对PCBs/2，4-DCP复合污染物的耐受能力显著高于非转基因株系。在较短的时间内，PCBs的降解能力较强，对2，4-DCP的去除率较高.证实了表达BphC.B是一种可行的策略，有助于实现PCBs和2，4-DCP混合污染土壤的植物修复。
Polychlorinated biphenyls (PCBs) and 2,4-dichlorophenol (2,4-DCP) generally led to mixed contamination of soils as a result of commercial and agricultural activities. Their accumulation in the environment poses great risks to human and animal health. Therefore, the effective strategies for disposal of these pollutants are urgently needed. In this study, genetic engineering to enhance PCBs/2,4-DCP phytoremediation is a focus. We cloned the 2,3-dihydroxybiphenyl-1,2-dioxygenase (BphC.B) from a soil metagenomic library, which is the key enzyme of aerobic catabolism of a variety of aromatic compounds, and then it was expressed in alfalfa driven by CaMV 35S promoter usingAgrobacterium-mediated transformation. Transgenic line BB11 was selected out through PCR, Western blot analysis and enzyme activity assays. Its disposal and tolerance to both PCBs and 2,4-DCP were examined. The tolerance capability of transgenic line BB11 towards complex contaminants of PCBs/2,4-DCP significantly increased compared with non-transgenic plants. Strong dissipation of PCBs and high removal efficiency of 2,4-DCP were exhibited in a short time. It was confirmed expressing BphC.B would be a feasible strategy to help achieving phytoremediation in mixed contaminated soils with PCBs and 2,4-DCP.