Effect of benzalkonium chloride addition to EDTA on attachment and proliferation of dental pulp stem cells on dentin and on transforming growth factor-β1 release
Effect of benzalkonium chloride addition to EDTA on attachment and proliferation of dental pulp stem cells on dentin and on transforming growth factor-β1 release
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DOI：
10.1007/s10266-020-00545-5
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发表时间：
2020-08-07
期刊：
影响因子：
2.5
通讯作者：
Parashos, Peter
中科院分区：
文献类型：
--
作者：
Eren, Selen Kucukkaya;Zirh, Elham Bahador;Parashos, Peter
目的：研究苯扎氯铵(BAC)与乙二胺四乙酸(EDTA)联合作用对牙本质上转化生长因子-β1(TGF-β1)释放及牙髓干细胞(DPSCs)贴壁和增殖的影响。制备268个标准人牙本质圆盘，在1.5%次氯酸钠中浸泡5min。用蒸馏水冲洗圆盘，随机分为7组。对照组不做进一步处理。剩余的圆盘浸泡在以下溶液中：17%EDTA或17%EDTA+0.008%BAC，分别浸泡1、5或10分钟，用蒸馏水冲洗。由于对有细胞和无细胞的盘进行了转化生长因子-β1释放试验，因此将DPSCs种植在部分盘中。分别用乳酸脱氢酶活性和WST-1法检测DPSCs在牙本质盘上的附着和增殖情况。扫描电子显微镜观察细胞形态。用双抗体夹心法测定细胞内转化生长因子-β1的释放。数据分析采用带Bonferroni校正的三因素方差分析和双向方差分析。两种EDTA溶液均可促进DPSCs的附着和增殖(p<；0.05)，但两者之间无显著差异(p>；0.05)。两种EDTA溶液作用时间对细胞贴壁、增殖和转化生长因子-β1的释放均无影响(p>；0.05)。对照组和实验组之间的转化生长因子-β1的释放无显著差异(p>；0.05)。无论牙本质盘是否种植细胞，牙本质盘释放的转化生长因子-β1的量是相似的(p>；0.05)。两种EDTA溶液处理牙本质均不影响转化生长因子-β1的释放量，但两种EDTA溶液均可促进细胞在牙本质表面的附着和增殖，而与暴露时间无关。
To investigate the effect of benzalkonium chloride (BAC) addition to ethylenediaminetetraacetic acid (EDTA) on transforming growth factor-beta 1 (TGF-beta 1) release, as well as attachment and proliferation of dental pulp stem cells (DPSCs) on dentin. A total of standard 268 human dentin disks were prepared and immersed in 1.5% sodium hypochlorite (NaOCl) for 5 min. The disks were rinsed with distilled water and randomly divided into seven groups. In control group, the disks received no further treatment. The remaining disks were immersed in following solutions: 17% EDTA or 17% EDTA + 0.008% BAC for 1, 5 or 10 min and rinsed with distilled water. DPSCs were seeded in part of the disks since the TGF-beta 1 release assay was performed with disks with and without cells. The attachment and proliferation of DPSCs on dentin disks were analyzed using lactate dehydrogenase activity and WST-1 assays, respectively. The cell morphology was observed by scanning electron microscopy. The release of TGF-beta 1 was quantified using ELISA. Data were analyzed using three- and two-way analysis of variance with Bonferroni corrections. Both EDTA solutions increased the attachment and proliferation of DPSCs (p < .05) while there was no significant difference between them (p > .05). The exposure time of both EDTA solutions had no influence on cell attachment, proliferation and TGF-beta 1 release (p > .05). There was no significant difference in TGF-beta 1 release between the control and experimental groups (p > .05). The amount of released TGF-beta 1 from dentin disks was similar whether or not they were seeded with cells (p > .05). Dentin treatment with either of the EDTA solutions had no effect on the amount of TGF-beta 1 release while both EDTA solutions improved cell attachment and proliferation on dentin surface regardless of exposure time.