OPTIMIZING AND CHARACTERIZING ALIGNMENT OF ORIENTED LIPID BILAYERS CONTAINING GRAMICIDIN-D
OPTIMIZING AND CHARACTERIZING ALIGNMENT OF ORIENTED LIPID BILAYERS CONTAINING GRAMICIDIN-D
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DOI：
10.1016/s0006-3495(90)82536-4
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发表时间：
1990-02-01
影响因子：
3.4
通讯作者：
CROSS, TA
中科院分区：
文献类型：
--
作者：
MOLL, F;CROSS, TA
31 P NMR光谱和光学显微镜已被用来表征样品短杆菌肽D在定向脂双层。用光学显微镜观察到的缺陷结构与~（31）P核磁共振谱的特征之间进行了关联。使用这些技术改进了样品制备方案，以实现双层正常样品的最小分散和最少量的未取向样品。短杆菌肽与二肉豆蔻酰磷脂酰胆碱的摩尔比、水合程度和共增溶溶剂系统被用作方案变量。水化程度和溶剂体系对样品取向有显著影响，但摩尔比对取向影响不大。然而，31 P化学位移各向异性是非常敏感的摩尔比，可以用来作为一种原位方法来确定的摩尔比。
31P NMR spectroscopy and optical microscopy have been used to characterize samples of gramicidin D in oriented lipid bilayers. Correlations have been made between the defect structures observed under crossed polarizers by optical microscopy and characteristic features of 31P NMR spectra. The sample preparation protocol has been improved using these techniques to achieve minimal dispersion of the bilayer normal and minimal amounts of unoriented sample. The molar ratio of gramicidin to dimyristoyl-phosphatidylcholine, the extent of hydration, and the cosolubilizing solvent system were used as the protocol variables. While hydration level and solvent system had profound effects on the sample orientation the molar ratio did not. However, the 31P chemical shift anisotropy is very sensitive to the molar ratio and can be used as an in situ method for determining the molar ratio.