An in vitro model of infection of human biliary epithelial cells by Cryptosporidium parvum
An in vitro model of infection of human biliary epithelial cells by Cryptosporidium parvum
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DOI：
10.1086/593695
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发表时间：
1997-05-01
影响因子：
6.4
通讯作者：
Ward, HD
中科院分区：
文献类型：
--
作者：
Verdon, R;Keusch, GT;Ward, HD
免疫抑制宿主中的小隐孢子虫感染常常因胆道受累而变得复杂，最近生产的人胆管上皮细胞系被用来开发胆管隐孢子虫病的体外模型。通过 LFA 和 ELISA 检测微小隐孢子虫卵囊的感染，并通过透射电子显微镜证实。单层接种10(4)至5×10(5)卵囊/孔导致感染呈剂量依赖性增加，时程实验表明，接种后18-24小时寄生阶段数量最多，50和100μg/mL浓度的胆汁显着增强感染，400μg/mL巴龙霉素抑制感染。通过使用 IFA 或 ELISA，可以持续实现和监测微小念珠菌对人胆管细胞的感染。该系统将用于评估微小隐孢子虫感染的机制以及对胆道隐孢子虫病治疗药物的反应。
Cryptosporidium parvum infection in the immunosuppressed host is frequently complicated by biliary tract involvement, The recent production of human biliary epithelial cell lines was exploited to develop an in vitro model of biliary cryptosporidiosis. Infection with C. parvum oocysts was detected by LFA and ELISA and confirmed by transmission electron microscopy. Inoculation of monolayers with 10(4) to 5 x 10(5) oocysts/well resulted in a dose-dependent increase in infection, Time-course experiments showed that the number of parasitic stages was maximal at 18-24 h after inoculation, Infection was significantly enhanced by bile at concentrations of 50 and 100 mu g/mL and inhibited by 400 mu g/mL paromomycin. Infection of human biliary cells with C. parvum can be consistently achieved and monitored by use of IFA or ELISA. This system will be of use in evaluating mechanisms of C. parvum infection and response to therapeutic agents in biliary cryptosporidiosis.