Effect of Melanotan-II on Brain Fos Immunoreactivity and Oxytocin Neuronal Activity and Secretion in Rats.
Effect of Melanotan-II on Brain Fos Immunoreactivity and Oxytocin Neuronal Activity and Secretion in Rats.
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Melanotan-II 对大鼠脑 Fos 免疫反应性和催产素神经元活性和分泌的影响。
DOI：
10.1111/jne.12454
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发表时间：
2017
影响因子：
3.2
通讯作者：
Paiva L
中科院分区：
文献类型：
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作者：
Paiva L
黑素皮质激素刺激中枢催产素系统，参与调节社会行为。中枢催产素的改变与自闭症等神经系统疾病有关，黑素皮质素已被提议用于治疗。在本研究中，我们研究了黑素皮质激素激动剂黑素素- II （MT - II）如何影响大鼠催产素神经元的活性和分泌。得到的结果表明：MT‐II可显著诱导下丘脑视上核（SON）和室旁核（PVN）的大细胞神经元中Fos的表达，而优先使用黑素皮质素拮抗剂SHU‐9119可减弱这种反应。通过微透析测量，经鉴定的大细胞神经元的电生理记录显示，MT - II的使用增加了催产素神经元的放电率，但没有触发SON内的体树突催产素释放。我们的数据表明，在。但鼻内注射MT - II后，SON的大细胞神经元活性增加。由于先前的研究表明，直接应用黑素皮质激素激动剂会抑制SON催产素神经元，因此其作用。MT‐II可能至少部分间接介导，可能通过激活尾侧脑干的输入，其中MT‐II也增加了Fos的表达。
Melanocortins stimulate the central oxytocin systems that are involved in regulating social behaviours. Alterations in central oxytocin have been linked to neurological disorders such as autism, and melanocortins have been proposed for therapeutic treatment. In the present study, we investigated how systemic administration of melanotan‐II (MT‐II), a melanocortin agonist, affects oxytocin neuronal activity and secretion in rats. The results obtained show thati.v., but not intranasal, administration of MT‐II markedly induced Fos expression in magnocellular neurones of the supraoptic (SON) and paraventricular nuclei (PVN) of the hypothalamus, and this response was attenuated by priori.c.v.administration of the melanocortin antagonist, SHU‐9119. Electrophysiological recordings from identified magnocellular neurones of the SON showed thati.v.administration of MT‐II increased the firing rate in oxytocin neurones but did not trigger somatodendritic oxytocin release within the SON as measured by microdialysis. Our data suggest that, afteri.v., but not intranasal, administration of MT‐II, the activity of magnocellular neurones of the SON is increased. Because previous studies showed that SON oxytocin neurones are inhibited in response to direct application of melanocortin agonists, the actions ofi.v.MT‐II are likely to be mediated at least partly indirectly, possibly by activation of inputs from the caudal brainstem, where MT‐II also increased Fos expression.