Triphenyltetrazolium staining of irreversible ischemic injury following coronary artery occlusion in rats.
Triphenyltetrazolium staining of irreversible ischemic injury following coronary artery occlusion in rats.
复制标题
DOI：
--
复制
发表时间：
1985-12
期刊：
影响因子：
--
通讯作者：
M. T. Vivaldi;R. Kloner;F. Schoen
中科院分区：
文献类型：
--
作者：
M. T. Vivaldi;R. Kloner;F. Schoen
本研究的目的是氯化三苯基四氮唑（TTC）的心肌梗死的组织化学染色，以确定已建立的梗死的大小的准确性，以确定在梗死演变过程中的染色缺陷的发展的时间过程，并确定临床相关的自溶对早期梗死的可检测性的影响。将左冠状动脉闭塞后切除的大鼠心脏的横切面在TTC中于37 ℃孵育5分钟。48小时后，TTC和常规组织学研究（H）平面测量确定的梗死面积（左心室百分比[LV%]）相关性良好：TTC（LV%）= 1.04 × H（LV%）- 2.94（r = 0.98; P <0.001）。在闭塞30分钟后在50%的心脏中检测到缺血区染色缺陷，在闭塞3小时或更长时间的所有心脏中注意到。在环境温度下原位自溶6小时或在4 ℃下自溶24或72小时，大体染色对比度没有降低。总之，TTC可以可靠地定量完全梗死的大小。TTC通常在大鼠冠状动脉闭塞后30分钟检测到缺血性损伤，在所有心脏中检测到3-6小时。在模拟临床自溶过程中保留TTC的细胞界限。
The objectives of this study of triphenyltetrazolium chloride (TTC) gross histochemical staining of myocardial infarcts were to determine the accuracy of sizing of established infarcts, to determine the time course of development of staining defects during infarct evolution, and to determine the effect of clinically relevant autolysis on the detectability of early infarcts. Transverse sections of excised rat hearts after left coronary artery occlusion were incubated in TTC for 5 minutes at 37 C. After 48 hours, planimetrically determined infarct size (percent left ventricle [LV%]) by TTC and by conventional histologic study (H) correlated well: TTC (LV%) = 1.04 X H(LV%) - 2.94 (r = 0.98; P less than 0.001). Ischemic zone staining defects, detected in 50% of hearts 30 minutes after occlusion, were noted in all hearts occluded 3 hours or longer. Gross staining contrast was not diminished by in situ autolysis for 6 hours at ambient temperature or for 24 or 72 hours at 4 C. In conclusion, TTC reliably permits quantitation of the size of completed infarcts. TTC often detects ischemic injury as early as 30 minutes after coronary occlusion in the rat and in all hearts by 3-6 hours. Infarct demarcation by TTC is preserved during simulated clinical autolysis.