Direct Electrochemistry of Glucose Oxidase Immobilized on Chitosan-gold Nanoparticle Composite Film on Glassy Carbon Electrodes and Its Biosensing Application
Direct Electrochemistry of Glucose Oxidase Immobilized on Chitosan-gold Nanoparticle Composite Film on Glassy Carbon Electrodes and Its Biosensing Application
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DOI：
10.1002/cjoc.200890226
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发表时间：
2008-07
影响因子：
5.4
通讯作者：
Qinglin Sheng;Yuesheng Shen;Hongfang Zhang;Jianbin Zheng
中科院分区：
文献类型：
--
作者：
Qinglin Sheng;Yuesheng Shen;Hongfang Zhang;Jianbin Zheng
实现了葡萄糖氧化酶（GOx）在壳聚糖（CHIT）和金纳米粒子（Au NP）复合基质上的直接电化学反应。采用循环伏安法和电化学阻抗法对改性电极进行了表征。在去氧缓冲溶液中，氧化石墨烯- au NP-CHIT复合膜的循环伏安法显示出一对表现良好的氧化还原峰，属于氧化还原反应，证实了氧化石墨烯在复合膜上的有效固定。电子转移速率常数估计为15.6 s−1，表明GOx氧化还原中心和电极之间的电子转移率很高。CHIT和Au NP的结合还促进了复合膜中GOx的稳定性，并保留了其生物活性，可能在葡萄糖测定中具有潜在的应用前景。表观Michaelis-Menten常数为10.1 mmol·L−1。此外，所提出的生物传感器可用于测定人体血浆样品中的葡萄糖。
The direct electrochemistry of glucose oxidase (GOx) immobilized on a composite matrix based on chitosan (CHIT) and Au nanoparticles (Au NP) underlying on a glassy carbon electrode was achieved. The cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the modified electrode. In deaerated buffer solutions, the cyclic voltammetry of the composite films of GOx-Au NP-CHIT showed a pair of well-behaved redox peaks that were assigned to the redox reaction of GOx, confirming the effective immobilization of GOx on the composite film. The electron transfer rate constant was estimated to be 15.6 s−1, indicating a high electron transfer between the GOx redox center and electrode. The combination of CHIT and Au NP also promoted the stability of GOx in the composite film and retained its bioactivity, which might have the potential application to glucose determination. The calculated apparent Michaelis-Menten constant was 10.1 mmol·L−1. Furthermore, the proposed biosensor could be used for the determination of glucose in human plasma samples.