Characterizing multilaminated hydrogels with spatially varying network structure and solute loading using confocal laser scanning microscopy
Characterizing multilaminated hydrogels with spatially varying network structure and solute loading using confocal laser scanning microscopy
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DOI：
10.1016/j.actbio.2006.11.006
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发表时间：
2007-07-01
影响因子：
9.7
通讯作者：
Anseth, Kristi S.
中科院分区：
文献类型：
--
作者：
Watkins, Andrew W.;Southard, Stephanie L.;Anseth, Kristi S.
通过光聚合技术制备了具有不同空间溶质负载和由聚甲基丙烯酸羟乙酯（PHEMA）和聚乙二醇（PEG）组成的网状结构的水凝胶。以低分子量荧光染料为模型药物，利用共聚焦激光扫描显微镜（CLSM）对释放过程中的分布进行了无创实时表征。为了进行比较，基于菲克扩散理论的理论建模与实验工作相结合，以确定与预期行为的任何偏差，并指导未来装置的开发。在仅由PHEMA组成的多层板中，染料在释放过程中的分布演变和累积释放曲线与理论预测数据吻合良好，表明扩散是连续的，层间界面障碍不明显。然而，在由PHEMA和PEG交替层组成的装置中，实验观察到的浓度分布和释放速率与预测的行为存在差异，这表明扩散的界面阻碍可能是由于相互穿透网络的形成。最后，监测了两种染料以不同速率从PEG/PHEMA多层板同时释放，以证明CLSM在理解get设备中溶质分布的复杂时间变化方面的有用性。由爱思唯尔有限公司代表Acta Materialia公司出版。
Multilaminated controlled release devices were formed through photopolymerization techniques to produce hydrogels with spatially varying solute loadings and network structures composed of poly(hydroxyl ethyl methacrylate) (PHEMA) and poly(ethylene glycol) (PEG). Using low molecular weight fluorescent dyes as model drugs, the distribution profiles were characterized non-invasively in pseudo-real-time with confocal laser scanning microscopy (CLSM) during release studies. For comparison, theoretical modeling based on Fickian diffusion theory was performed in conjunction with experimental work to identify any deviations from expected behavior and to guide in the development of future devices. In multilaminates composed of only PHEMA, the evolution of dye distribution during release and cumulative release profiles agreed well with theoretically predicted data, indicating continuity of diffusion and insignificant interfacial hindrance between layers. However, in devices composed of alternating layers of PHEMA and PEG, differences from predicted behavior were experimentally observed in both concentration profiles and release rates, suggesting interfacial obstruction of diffusion, possibly due to the formation of interpenetrating networks. Finally, the simultaneous release of two dyes at different rates from a PEG/PHEMA multilaminate was monitored to demonstrate the usefulness of CLSM in understanding the complex temporal changes in solute distributions in get devices. Published by Elsevier Ltd. on behalf of Acta Materialia Inc.