LIGAND-INDUCED RAPID REDISTRIBUTION OF LYSOSOMES IS TEMPORALLY DISTINCT FROM ENDOSOME TRANSLOCATION
LIGAND-INDUCED RAPID REDISTRIBUTION OF LYSOSOMES IS TEMPORALLY DISTINCT FROM ENDOSOME TRANSLOCATION
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DOI：
10.1038/304738a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
ALBERTINI, DF
中科院分区：
文献类型：
--
作者：
HERMAN, B;ALBERTINI, DF
当许多配体与细胞表面受体结合时，配体-受体复合物通过一种称为受体介导的内吞作用的过程通过网格蛋白包裹的凹坑内化1,2。内化于内吞囊泡或内体内的配体的细胞质命运是可变的。例如，母体免疫球蛋白通过新生儿肠上皮细胞的细胞质运输，并在基底外侧表面胞外化3。然而，其他配体由于被递送到细胞的溶酶体室而被降解2,4 - 6。虽然核内体易位到细胞中心的高尔基区似乎是一种普遍现象，可能与溶酶体的配体降解有关7,8，而且核内体和溶酶体在细胞质内进行跳跃运动8,9，但这两种结构之间相互作用的空间控制尚不清楚。为了解决这个问题，我们已经开始研究内核体和溶酶体在细胞内的时空分布。利用一种新的荧光显微方法，我们现在已经能够同时观察活细胞中的内核体和溶酶体种群。我们的研究结果表明，不同类型的配体与细胞表面的结合会迅速诱导溶酶体的特异性重新定位；溶酶体向细胞高尔基体区的迁移先于内体向同一区域的易位。
When many ligands bind to cell-surface receptors, ligand–receptor complexes are internalized via clathrin coated pits by a process called receptor-mediated endocytosis1,2. The cytoplasmic fate of ligands internalized within endocytic vesicles or endosomes is variable. For example, maternal immunoglobulins are transported through the cytoplasm of neonatal intestinal epithelial cells and are exocytosed at the basolateral surface3. However, other ligands are degraded as a result of their delivery to the lysosomal compartment of cells2,4–6. Although the translocation of endosomes to the Golgi region in the cell centre seems to be a general phenomenon presumably coupled to ligand degradation by lysosomes7,8and endosomes and lysosomes undergo saltatory movements within the cytoplasm8,9, the spatial control of interaction between the two structures is not understood. To address this problem we have begun to examine the spatial and temporal intracellular distribution of endosomes and lysosomes. Utilizing a new fluorescent microscopic approach10, we have now been able simultaneously to visualize endosome and lysosome populations in living cells. Our results suggest that a specific relocation of lysosomes is rapidly induced upon binding of different types of ligands to the cell surface; this migration of lysosomes to the Golgi region of the cell precedes the translocation of endosomes into the same area.