Kinetic Template-Guided Tethering of Fragments
Kinetic Template-Guided Tethering of Fragments
复制标题
动力学模板引导的碎片系链
DOI：
10.1002/cmdc.201200404
复制
发表时间：
2012-12-01
期刊：
影响因子：
3.4
通讯作者：
Mann, David J.
中科院分区：
文献类型：
--
作者：
Nonoo, Rebecca H.;Armstrong, Alan;Mann, David J.
系链的鸟：描述了一种用于定点发现弱结合到定义的蛋白质表面的低分子量片段的拴系策略。暴露于溶剂的蛋白质硫醇在缀合物加成反应中捕获丙烯酰胺修饰的片段，该缀合物加成反应需要模板以产生可测量量的蛋白质片段加合物，其可以通过质谱法快速鉴定。
Birds of a tether: A tethering strategy for the site-directed discovery of low-molecular-weight fragments that bind weakly to defined protein surfaces is described. A solvent-exposed protein thiol captures acrylamide-modified fragments in a conjugate addition reaction that requires a template to produce a measureable quantity of protein-fragment adduct, which can be rapidly identified by mass spectrometry.