Immunoradiometric assay of active renin in human plasma: comparison with plasma renin activity.
Immunoradiometric assay of active renin in human plasma: comparison with plasma renin activity.
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人血浆中活性肾素的免疫放射测定：与血浆肾素活性的比较。
DOI：
10.3109/10641968709158991
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
A. Rappelli
中科院分区：
文献类型：
--
作者：
P. Dessì‐Fulgheri;F. Cocco;N. Glorioso;F. Bandiera;P. Madeddu;A. Rappelli
用单克隆抗体直接测定高血压患者血浆活性肾素（IRMA），并与传统的PRA法进行比较。使用两种单克隆抗体：3E 8和4G 1。第一个被耦合到可磁化的珠子，并用于捕获血浆中的活性和非活性的肾素。将第二抗体4G 1碘化并用于检测3E 8捕获的活性肾素。在PRA值高于2 ng/ml/h的血浆样本中，两种方法之间的相关系数非常高（r = 0.98，p <0.001）;在低肾素样本（PRA低于2 ng/ml/h）中，未发现显著相关性（r = 0.12 n.s.）。用胰蛋白酶活化失活的肾素前后分别进行PRA和IRMA，计算出失活的肾素占总肾素的百分比分别为86.8%和84%。直接单克隆抗体法测定活性肾素，可有效地采用，与传统的PRA程序，在研究临床和病理生理方面的肾素-血管紧张素系统。
The direct measurement of active renin with monoclonal antibodies (IRMA) in plasma from hypertensive patients was compared with the traditional PRA method. Two monoclonal antibodies were used: 3E8 and 4G1. The first was coupled to magnetizable beads and was used to trap both active and inactive renin from plasma. The second antibody, 4G1, was iodinated and used to detect active renin trapped by 3E8. The correlation coefficient between the two methods was very high (r = 0.98, p less than 0.001) in plasma samples whose PRA values were higher than 2 ng/ml/h; in low renin samples (PRA lower than 2 ng/ml/h) no significant correlation was found (r = 0.12 n.s.). When PRA and IRMA were performed before and after trypsin activation of inactive renin, the percentage of inactive over total renin was 86.8% and 84% as calculated with PRA and IRMA respectively. The direct monoclonal antibodies method for measuring active renin can be usefully adopted, in conjunction with the traditional PRA procedure, in studying both clinical and pathophysiological aspects of the renin-angiotensin system.