Dissecting in vitro host cell infection by Plasmodium sporozoites using flow cytometry
Dissecting in vitro host cell infection by Plasmodium sporozoites using flow cytometry
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DOI：
10.1111/j.1462-5822.2007.01032.x
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发表时间：
2008-01-01
影响因子：
3.4
通讯作者：
Mota, Maria M.
中科院分区：
文献类型：
--
作者：
Prudencio, Miguel;Rodrigues, Cristina D.;Mota, Maria M.
疟疾肝脏阶段的研究一直受到实验方法的限制，需要有效地解剖和量化肝细胞感染疟原虫。在这里，我们报告使用流式细胞术，结合GFP表达疟原虫子孢子，以评估构成一个成功的疟疾肝感染的各个步骤：细胞穿越，肝细胞入侵和肝细胞内寄生虫的发展。我们表明，这种快速，有效和廉价的方法可以用来克服目前的局限性，在独立的量化这些步骤，促进常规或大规模的研究宿主-病原体分子相互作用。
The study of the liver stage of malaria has been hampered by limitations in the experimental approaches required to effectively dissect and quantify hepatocyte infection by Plasmodium. Here, we report on the use of flow cytometry, in conjunction with GFP-expressing Plasmodium sporozoites, to assess the various steps that constitute a successful malaria liver infection: cell traversal, hepatocyte invasion and intrahepatocyte parasite development. We show that this rapid, efficient and inexpensive method can be used to overcome current limitations in the independent quantification of those steps, facilitating routine or large-scale studies of host-pathogen molecular interactions.