Synthetic molecular recognition nanosensor paint for microalbuminuria
Synthetic molecular recognition nanosensor paint for microalbuminuria
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DOI：
10.1038/s41467-019-11583-1
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发表时间：
2019-08-09
影响因子：
16.6
通讯作者：
Heller, Daniel A.
中科院分区：
文献类型：
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作者：
Budhathoki-Uprety, Januka;Shah, Janki;Heller, Daniel A.
微量白蛋白尿是多种心血管、代谢和其他疾病（如糖尿病、高血压、动脉粥样硬化和癌症）的重要临床标志物。微量白蛋白尿的准确检测依赖于尿液中白蛋白的定量，通常通过免疫比浊测定进行；然而，与许多基于抗体的评估一样，这种方法可能不够稳健，无法在全球健康应用、即时检测或可穿戴设备中发挥作用。在这里，我们开发了一种使用合成分子识别的无抗体方法，通过构建聚合物来模拟脂肪酸与白蛋白的结合，并根据白蛋白晶体结构进行了解。被聚合物封装的单壁碳纳米管，作为转导元件，在与临床尿样中的白蛋白结合时产生光致发光的低色（蓝色）位移。这种复合物与丙烯酸材料结合在一起，形成了一种纳米传感器涂料，能够检测患者样本中的微量白蛋白尿，并包含一个足够强大的快速护理点传感器，可以在资源有限的环境中部署。
Microalbuminuria is an important clinical marker of several cardiovascular, metabolic, and other diseases such as diabetes, hypertension, atherosclerosis, and cancer. The accurate detection of microalbuminuria relies on albumin quantification in the urine, usually via an immunoturbidity assay; however, like many antibody-based assessments, this method may not be robust enough to function in global health applications, point-of-care assays, or wearable devices. Here, we develop an antibody-free approach using synthetic molecular recognition by constructing a polymer to mimic fatty acid binding to the albumin, informed by the albumin crystal structure. A single-walled carbon nanotube, encapsulated by the polymer, as the transduction element produces a hypsochromic (blue) shift in photoluminescence upon the binding of albumin in clinical urine samples. This complex, incorporated into an acrylic material, results in a nanosensor paint that enables the detection of microalbuminuria in patient samples and comprises a rapid point-of-care sensor robust enough to be deployed in resource-limited settings.