Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae.
Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae.
复制标题
DOI：
10.15698/mic2014.09.164
复制
发表时间：
2014-08-27
期刊：
影响因子：
--
通讯作者：
Chaves SR
中科院分区：
文献类型：
--
作者：
Rego A;Duarte AM;Azevedo F;Sousa MJ;Côrte-Real M;Chaves SR
乙酸引发酿酒酵母细胞凋亡，类似于哺乳动物细胞凋亡。为了揭示这一过程的新调节因子，我们分析了MAPK信号传导受损是否会影响乙酸诱导的细胞凋亡，并发现交配信息素反应，特别是细胞壁完整性途径是主要介质，尤其是后者，我们对此进行了进一步的表征。筛选该途径的下游效应子，即转录因子 Rlm1p 的靶标，强调细胞壁重塑的减少对于乙酸抗性特别重要。因此，细胞表面动力学的调节成为增加乙酸抗性的有力策略，具有在使用酵母的工业发酵中的潜在应用，以及在生物医学中利用结直肠癌细胞对肠道丙酸杆菌产生的乙酸诱导的细胞凋亡的更高敏感性。
Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wall integrity pathways were the major mediators, especially the latter, which we characterized further. Screening downstream effectors of this pathway, namely targets of the transcription factor Rlm1p, highlighted decreased cell wall remodeling as particularly important for acetic acid resistance. Modulation of cell surface dynamics therefore emerges as a powerful strategy to increase acetic acid resistance, with potential application in industrial fermentations using yeast, and in biomedicine to exploit the higher sensitivity of colorectal carcinoma cells to apoptosis induced by acetate produced by intestinal propionibacteria.