Methylation of Dickkopf-3 as a prognostic factor in cirrhosis-related hepatocellular carcinoma.
Methylation of Dickkopf-3 as a prognostic factor in cirrhosis-related hepatocellular carcinoma.
复制标题
DOI：
10.3748/wjg.v16.i6.755
复制
发表时间：
2010-02
影响因子：
4.3
通讯作者：
Bin Yang;Z. Du;Yingtang Gao;Cheng Lou;Shi-guang Zhang;T. Bai;Yi-jun Wang;Wen-qin Song
中科院分区：
文献类型：
--
作者：
Bin Yang;Z. Du;Yingtang Gao;Cheng Lou;Shi-guang Zhang;T. Bai;Yi-jun Wang;Wen-qin Song
目的探讨Dickkopf（DKK）家族基因甲基化的发生率、发生时间及其在肝癌发生中的临床意义。方法对115例肝组织标本进行DKK家族基因甲基化定量分析，其中包括50对原发性肝细胞癌（HCC）组织和15例肝硬化活检组织标本。结果肝癌组织中DKK 3甲基化水平显著高于癌旁正常肝组织（P <1%），DKK 3甲基化水平较低者（PMR <或= 1%）显著降低（P = 0.0255）。多因素考克斯分析显示，甲基化DKK 3与肝癌患者生存期缩短显著独立相关（相对危险度= 2.527，95% CI：1.063-6.008，P = 0.036）。结论DKK 3基因甲基化是HCC早期恶变和进展的重要事件，可作为HCC危险性评估的预后指标。
AIM To investigate the prevalence and time of Dickkopf (DKK) family methylation and its clinical significance in hepatocarcinogenesis. METHODS Methylation of DKK family genes was quantitatively analyzed in 115 liver tissue samples, including 50 pairs of primary hepatocellular carcinoma (HCC) and matched noncancerous cirrhotic tissue samples, as well as 15 liver cirrhosis biopsy samples. RESULTS The methylation level of DKK3 was significantly higher in HCC tissue samples than in matched noncancerous cirrhotic tissue samples (P 1%) was significantly shorter than that of those with a lower DKK3 methylation level (PMR < or = 1%) (P = 0.0255). Multivariate Cox analysis indicated that methylated DKK3 was significantly and independently related with a shorter survival time (relative risk = 2.527, 95% CI: 1.063-6.008, P = 0.036) of HCC patients. CONCLUSION Methylation of DKK3 is an important event in early malignant transformation and HCC progression, and therefore might be a prognostic indicator for risk assessment of HCC.