Infection by Agrobacterium tumefaciens increased the resistance of leaf explants to selective agents in carnation (Dianthus caryophyllus L. and D. chinensis)
Infection by Agrobacterium tumefaciens increased the resistance of leaf explants to selective agents in carnation (Dianthus caryophyllus L. and D. chinensis)
复制标题
根癌农杆菌的感染增加了康乃馨（石竹和石竹）叶外植体对选择剂的抗性
DOI：
10.1016/j.plantsci.2004.07.030
复制
发表时间：
2005
期刊：
影响因子：
5.2
通讯作者：
M. Welander
中科院分区：
文献类型：
--
作者：
Song Zhang;Li;Xueyuan Li;A. Ahlman;M. Welander
选择是植物转化的关键，选择压力是影响转化效率的重要因素之一。在某些情况下，选择剂的合适浓度是根据再生试验选择的，细菌感染对外植体对选择剂的抗性的潜在影响可能被忽略了。为了评价农杆菌感染对外植体对选择剂抗性的影响，我们对两个香石竹品种进行了两个菌株（EHA 101和C58 C1）感染的几个实验，只携带一个辅助质粒，根癌农杆菌。选择剂为卡那霉素、甘露糖和膦丝菌素（PPT）。结果表明，细菌感染确实增加了叶片外植体对选择剂的耐受性，并且这种增加是基因型依赖的。一般来说，在没有细菌感染的情况下，芽形成的最高浓度为卡那霉素25 - 50 mgl − 1，甘露糖3 - 4 gl − 1，PPT 0 mgl − 1，这取决于基因型。在细菌感染后，根据品种的不同，在100 mgl − 1卡那霉素、4 - 6 gl − 1甘露糖或1 - 2 mgl − 1 PPT下观察到芽的形成。这表明，菌株和基因型之间在芽形成方面存在着相互作用。本研究的结果表明，细菌感染可以提高植物对选择剂的抗性，在确定转化选择剂的临界浓度阈值时应考虑这一点。
Selection is critical in plant transformation and the selection pressure is one of the most important factors affecting transformation efficiency. In some cases, a suitable concentration for selective agents is chosen based on regeneration tests and the potential influence of bacterial infection on the resistance of explants to selective agents has been probably neglected. In order to evaluate the effect of agrobacterial infection on the resistance of explants to selective agents, we performed several experiments on two carnation cultivars infected by two strains (EHA101 and C58C1), carrying only a helper plasmid, of Agrobacterium tumefaciens. The selective agents were kanamycin, mannose and phosphinothricin (PPT). The results have showed that the bacterial infection indeed increases the tolerance of leaf explants to the selective agents and this increase is genotype-dependent. In general, without the bacterial infection, the highest concentrations for shoot formation were 25–50mgl−1for kanamycin, 3–4gl−1for mannose and 0mgl−1for PPT depending on genotype. After the bacterial infection, shoot formation was observed at either 100mgl−1kanamycin, or 4–6gl−1mannose or 1–2mgl−1PPT depending on cultivar. It seems that there is an interaction between the bacterium strains and the genotypes in relation to shoot formation. The results in this study have provided evidence that bacterial infection can increase the plant resistance to selective agents, which should be considered when establishing a critical concentration threshold of selective agents for transformation.