The use of 'normal' and 'transformed' gynandromorphs in mapping the primordial germ cells and the gonadal mesoderm in Drosophila.
The use of 'normal' and 'transformed' gynandromorphs in mapping the primordial germ cells and the gonadal mesoderm in Drosophila.
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使用“正常”和“转化”雌雄同体来绘制果蝇原始生殖细胞和性腺中胚层的图谱。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
M. Holliger
中科院分区：
文献类型：
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作者：
W. Gehring;E. Wieschaus;M. Holliger
通过分析“正常”和“转化”雌雄同型的嵌合体模式，在果蝇胚胎中定位了原始生殖细胞和性腺中胚层。相对于成体角质层标记，生殖细胞被定位为最后面的结构，这与它们在胚泡胚胎中的已知位置一致。这些数据支持这样的假设，即雌雄同体图反映了胚胎原基的真实位置。由于胚胎期之后原始生殖细胞向前迁移，这些数据也表明该图谱实际上对应于胚胚期，而不是发育的后期。生殖盘位于生殖细胞前方和腹侧的单个正中原基上，性腺中胚层位于生殖盘的前面，也在胚胎的腹侧形成一个正中原基。生殖盘和性腺中胚层的原基大得不寻常，这可能反映了细胞谱系的某种不确定性，导致了图谱的‘扩展’。
The primordial germ cells and the gonadal mesoderm were mapped in the Drosophila embryo by analyzing the patterns of mosaicism in 'normal' and 'transformed' gynandromorphs. Relative to the adult cuticular markers the germ cells map as the posterior most structure, which coincides with their known location in the blastoderm embryo. These data support the hypothesis that the gynandromorph map reflects the real position of the primordia in the embryo. Since after the blastoderm stage the primordial germ cells migrate anteriorly these data also indicate that the map in fact corresponds to the blastoderm stage and not to a later stage of development. The genital disc maps at a single median primordium anterior and ventral to the germ cells, the gonadal mesoderm is located anterior to the genital disc and also forms a single median primordium on the ventral side of the embryo. The primordia for the genital disc and the gonadal mesoderm are unusually large in size, which presumably reflects some indeterminacy of the cell lineage leading to an 'expansion' of the map.