Elevated expression of protein biosynthesis genes in liver and muscle of hibernating black bears (Ursus americanus)
Elevated expression of protein biosynthesis genes in liver and muscle of hibernating black bears (Ursus americanus)
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DOI：
10.1152/physiolgenomics.90398.2008
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发表时间：
2009-04-01
影响因子：
4.6
通讯作者：
Barnes, Brian M.
中科院分区：
文献类型：
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作者：
Fedorov, Vadim B.;Goropashnaya, Anna V.;Barnes, Brian M.
Fedorov VB，Goropashnaya AV，Toien O，Stewart NC，Gracey AY，Chang C，Qin S，Pertea G，Quackenbush J，Showe LC，Showe MK，Boyer BB，巴恩斯BM.冬眠黑熊肝脏和肌肉中蛋白质合成基因的高表达。Physiol Genomics 37：108-118，2009.首次发表于2009年2月24日; doi：10.1152/physiolgenomics.90398.2008。我们使用专门为美洲熊开发的3，200个cDNA探针微阵列进行了大规模的基因表达筛选，以检测冬季冬眠期间与夏季采样的动物相比肝脏和骨骼肌中的表达差异。12个基因的表达，包括RNA结合蛋白基序3（Rbm 3），主要参与蛋白质的生物合成，诱导冬眠期间在肝脏和肌肉。基因本体论和基因集富集分析一致显示，在冬眠期间，肝脏和骨骼肌中的过表达基因高度显著地富集了蛋白质生物合成类别。冬眠黑熊转录组中蛋白质合成相关基因在转录水平上的协同诱导是一个显著的特征。这一发现意味着翻译的诱导，并提出了一种适应机制，有助于减少冬眠期间长时间不动的肌肉萎缩的独特能力。比较表达谱在熊的小型哺乳动物冬眠期间的转录变化，包括参与脂质代谢和碳水化合物合成的基因的诱导，以及参与尿素循环和解毒功能的基因在肝脏中的抑郁症的一般趋势。
Fedorov VB, Goropashnaya AV, Toien O, Stewart NC, Gracey AY, Chang C, Qin S, Pertea G, Quackenbush J, Showe LC, Showe MK, Boyer BB, Barnes BM. Elevated expression of protein biosynthesis genes in liver and muscle of hibernating black bears (Ursus americanus). Physiol Genomics 37: 108-118, 2009. First published February 24, 2009; doi:10.1152/physiolgenomics.90398.2008.-We conducted a large-scale gene expression screen using the 3,200 cDNA probe microarray developed specifically for Ursus americanus to detect expression differences in liver and skeletal muscle that occur during winter hibernation compared with animals sampled during summer. The expression of 12 genes, including RNA binding protein motif 3 (Rbm3), that are mostly involved in protein biosynthesis, was induced during hibernation in both liver and muscle. The Gene Ontology and Gene Set Enrichment analysis consistently showed a highly significant enrichment of the protein biosynthesis category by overexpressed genes in both liver and skeletal muscle during hibernation. Coordinated induction in transcriptional level of genes involved in protein biosynthesis is a distinctive feature of the transcriptome in hibernating black bears. This finding implies induction of translation and suggests an adaptive mechanism that contributes to a unique ability to reduce muscle atrophy over prolonged periods of immobility during hibernation. Comparing expression profiles in bears to small mammalian hibernators shows a general trend during hibernation of transcriptional changes that include induction of genes involved in lipid metabolism and carbohydrate synthesis as well as depression of genes involved in the urea cycle and detoxification function in liver.