Candida albicans biofilm-defective mutants
Candida albicans biofilm-defective mutants
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DOI：
10.1128/ec.4.8.1493-1502.2005
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发表时间：
2005-08-01
期刊：
影响因子：
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通讯作者：
Mitchell, AP
中科院分区：
文献类型：
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作者：
Richard, ML;Nobile, CJ;Mitchell, AP
生物膜的形成在许多微生物的生命周期和生存中起着关键作用。对于人类真菌病原体白色念珠菌，生物膜发育可以说是一种毒力性状，因为作为生物膜基质的医疗植入物是感染的重要风险因素。C.体外白念珠菌生物膜经历早期阶段（其中酵母细胞在基质中聚集）、中间阶段（其中产生假菌丝和菌丝细胞类型）和成熟阶段（其中细胞的持续生长伴随着细胞外基质的积累）。在这里，我们报告的结果筛选C。白色念珠菌生物膜缺陷突变体，其中NUP85、MDS3、KEM1和SUV 3中的纯合插入被发现阻断生物膜发育。共聚焦显微镜检查表明nup85，suv3和mds3突变引起早期阻滞，而kem1突变引起中期阻滞。所有的突变体在几种培养基中的菌丝生产都有缺陷。混合生物膜发展的分析表明，所有的突变体是有缺陷的，在生产的背景下的生物膜中的菌丝。因为所有的突变体在生物膜中的细胞保留方面都有缺陷，我们推断菌丝提供了稳定生物膜结构的粘附支架。
Biofilm formation plays a key role in the life cycles and subsistence of many microorganisms. For the human fungal pathogen Candida albicans, biofilm development is arguably a virulence trait, because medical implants that serve as biofilm substrates are significant risk factors for infection. The development of C. albicans biofilms in vitro proceeds through an early phase, in which yeast cells populate a substrate, an intermediate phase, in which pseudohyphal and hyphal cell types are produced, and a maturation phase, in which continued cell growth is accompanied by accumulation of an extracellular matrix. Here we report the results of a screen for C. albicans biofilm-defective mutants, in which homozygous insertions in NUP85, MDS3, KEM1, and SUV3 were found to block biofilm development. Confocal microscopic examination suggests that nup85, suv3, and mds3 mutations cause early-phase arrest, whereas the kem1 mutation causes intermediate-phase arrest. All of the mutants are defective in hypha production in several media. Analysis of mixed-biofilm development indicates that all of the mutants are defective in the production of hyphae in the context of a biofilm. Because all of the mutants are defective in the retention of cells in the biofilm, we infer that hyphae provide an adherent scaffold that stabilizes the biofilm structure.