Nucleolar localization of parafibromin is mediated by three nucleolar localization signals
Nucleolar localization of parafibromin is mediated by three nucleolar localization signals
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DOI：
10.1016/j.febslet.2007.09.050
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发表时间：
2007-10-30
期刊：
影响因子：
3.5
通讯作者：
Marsh, Deborah J.
中科院分区：
文献类型：
--
作者：
Hahn, Michael A.;Marsh, Deborah J.
Parafibromin是一种被认为由HRPT2编码的肿瘤抑制因子，与甲状旁腺肿瘤的发生有关。我们之前报道了在残基125-139处存在功能性的二部核定位信号（NLS）。我们现在证明，在76-92、192-194和393-409位点上，三种核仁定位信号（NoLS）介导了parafibromin的核仁定位。这些NoLS代表了基本氨基酸精氨酸和赖氨酸的簇，与其他核仁蛋白中发现的类似，也是nls的特征。虽然parafibromin的两部分NLS是核定位的主要决定因素，但它并不介导核定位。相比之下，这三种已确定的nols在核定位中只起次要作用，但对异丙基溴蛋白的核核定位至关重要。(C) 2007年欧洲生化学会联合会。Elsevier B.V.版权所有。
Parafibromin is a putative tumor suppressor encoded by HRPT2 and implicated in parathyroid tumorigenesis. We previously reported a functional bipartite nuclear localization signal (NLS) at residues 125-139. We now demonstrate that parafibromin exhibits nucleolar localization, mediated by three nucleolar localization signals (NoLS) at resides 76-92, 192-194 and 393-409. These NoLS represent clusters of basic amino acids arginine and lysine, similar to those found in other nucleolar proteins, as well as being characteristic of NLSs. While parafibromin's bipartite NLS is the primary determinant of nuclear localization, it does not mediate nucleolar localization. In contrast, the three identified NoLSs play only a minor role in nuclear localization, but are critical for the nucleolar localization of parafibromin. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.