KIF11 as a Potential Marker of Spermatogenesis Within Mouse Seminiferous Tubule Cross-sections
KIF11 as a Potential Marker of Spermatogenesis Within Mouse Seminiferous Tubule Cross-sections
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DOI：
10.1369/0022155419871027
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发表时间：
2019-08-19
影响因子：
3.2
通讯作者：
Iwabuchi, Kazuhisa
中科院分区：
文献类型：
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作者：
Hara-Yokoyama, Miki;Kurihara, Hidetake;Iwabuchi, Kazuhisa
未成熟生殖细胞的排列沿曲细精管轴沿着呈规律性和周期性变化，用于描述精子发生的进程。这种描述主要是基于单倍体精子细胞的顶体和核形态的变化。然而，这种标准不能应用于精子细胞分化停滞的病理条件下。在这种情况下，必须分析与减数分裂前生殖细胞分化相关的变化。在这里，我们发现，独特的双极电机蛋白，KIF 11（驱动蛋白-5/Eg5），在纺锤体形成过程中的有丝分裂和减数分裂的卵母细胞和早期胚胎的功能，在减数分裂前生殖细胞（精原细胞和精母细胞）中表达。因此，我们的目的是研究KIF 11是否可以用来描述不完全精子发生的进展。有趣的是，在单倍体精子细胞和Sertoli细胞中几乎没有观察到KIF 11的表达。KIF 11染色使我们能够评估减数分裂过程的进展，通过提供正常和精子发生停滞突变小鼠纺锤体形成的时间轴。因此，KIF 11有可能作为一个很好的标记来描述精子发生，即使在精子细胞发育的情况下。
The arrangement of immature germ cells changes regularly and periodically along the axis of the seminiferous tubule, and is used to describe the progression of spermatogenesis. This description is based primarily on the changes in the acrosome and the nuclear morphology of haploid spermatids. However, such criteria cannot be applied under pathological conditions with arrested spermatid differentiation. In such settings, the changes associated with the differentiation of premeiotic germ cells must be analyzed. Here, we found that the unique bipolar motor protein, KIF11 (kinesin-5/Eg5), which functions in spindle formation during mitosis and meiosis in oocytes and early embryos, is expressed in premeiotic germ cells (spermatogonia and spermatocytes). Thus, we aimed to investigate whether KIF11 could be used to describe the progression of incomplete spermatogenesis. Interestingly, KIF11 expression was barely observed in haploid spermatids and Sertoli cells. The KIF11 staining allowed us to evaluate the progression of meiotic processes, by providing the time axis of spindle formation in both normal and spermatogenesis-arrested mutant mice. Accordingly, KIF11 has the potential to serve as an excellent marker to describe spermatogenesis, even in the absence of spermatid development.