Identification of unannotated coding sequences and their physiological functions
Identification of unannotated coding sequences and their physiological functions
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未注释编码序列的鉴定及其生理功能
DOI：
10.1093/jb/mvac064
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Matsumoto Akinobu
中科院分区：
文献类型：
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作者：
Ichihara Kazuya;Nakayama Keiichi I;Matsumoto Akinobu
大多数蛋白质编码序列（CDS）是基于诸如足以编码至少100个氨基酸的产物的大小和从AUG起始密码子开始翻译的标准的预测序列。然而，最近基于核糖体分析和质谱的研究表明，只要非编码RNA被注释的几种RNA实际上被翻译以产生少于100个氨基酸的多肽，并且许多蛋白质从近同源起始密码子如CUG和GUG翻译。此外，对基因工程小鼠模型的研究表明，这些多肽和蛋白质有助于不同的生理过程。在这篇综述中，我们描述了最新的方法来识别未注释的CDS，并提供了它们的生理功能的例子。
Most protein-coding sequences (CDSs) are predicted sequences based on criteria such as a size sufficient to encode a product of at least 100 amino acids and with translation starting at an AUG initiation codon. However, recent studies based on ribosome profiling and mass spectrometry have shown that several RNAs annotated as long as noncoding RNAs are actually translated to generate polypeptides of fewer than 100 amino acids and that many proteins are translated from near-cognate initiation codons such as CUG and GUG. Furthermore, studies of genetically engineered mouse models have revealed that such polypeptides and proteins contribute to diverse physiological processes. In this review, we describe the latest methods for the identification of unannotated CDSs and provide examples of their physiological functions.