Caspase-1 from the silkworm, Bombyx mori, is involved in Bombyx mori nucleopolyhedrovirus infection
Caspase-1 from the silkworm, Bombyx mori, is involved in Bombyx mori nucleopolyhedrovirus infection
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来自家蚕 Bombyx mori 的 Caspase-1 参与家蚕核多角体病毒感染
DOI：
10.1515/znc-2016-0133
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发表时间：
2017-03-01
影响因子：
2
通讯作者：
Chen, Keping
中科院分区：
文献类型：
--
作者：
Wang, Qiang;Ju, Xiaoli;Chen, Keping
摘要Caspase-1是哺乳动物体内的效应半胱氨酸酶之一，在细胞凋亡中起着核心作用。然而，鳞翅目昆虫caspase-1，尤其是家蚕caspase-1(BM-caspase-1)还没有得到详细的研究。本研究通过BLAST搜索，从家蚕表达序列标签数据库中鉴定了BM-caspase-1。BM-caspase-1的开放阅读框包含879个核苷酸，编码293个氨基酸，预测的相对分子质量为33 kDa。Bm-caspase-1含有两个共同的caspase切割位点氨基酸基序：DEGDA和TETDG。半胱氨酸天冬氨酸酶活性测定表明，Ac-DEVD-PNA底物具有明显的蛋白降解活性。半定量聚合酶链式反应检测到BM-caspase-1在所有组织和发育阶段均有表达。更重要的是，Bm-caspase-1的表达水平在杆状病毒感染后增加，并在BmNPV抗性家蚕中上调。综上所述，这些结果表明BM-caspase-1在杆状病毒感染过程中起着重要作用。
Abstract Caspase-1 is one of the effector caspases in mammals that plays a central role in apoptosis. However, the lepidopteran caspase-1, especially the Bombyx mori caspase-1 (Bm-caspase-1), has not been investigated in detail. In this study, Bm-caspase-1 was identified from an expressed sequence tag database in B. mori by BLAST search. The open reading frame of Bm-caspase-1 contained 879 nucleotides and encoded 293 amino acids with a predicted molecular mass of 33 kDa. Bm-caspase-1 contained two consensus amino acid motifs of caspase cleavage sites, DEGDA and TETDG. Caspase activity assays revealed significant proteolytic activity of the Ac-DEVD-pNA substrate. Bm-caspase-1 can be detected in all tissues and developmental stages by a semi quantitative polymerase chain reaction assay. More importantly, the expression level of Bm-caspase-1 is increased upon baculovirus infection and up-regulated in BmNPV-resistant silkworms. Taken together, these results indicate that Bm-caspase-1 plays an important role during baculovirus infection.