Correspondence between plasma mevalonic acid levels and deuterium uptake in measuring human cholesterol synthesis.
Correspondence between plasma mevalonic acid levels and deuterium uptake in measuring human cholesterol synthesis.
复制标题
测量人体胆固醇合成时血浆甲羟戊酸水平与氘吸收之间的对应关系。
DOI：
10.1111/j.1365-2362.1992.tb01512.x
复制
发表时间：
1992
影响因子：
5.5
通讯作者：
Leitch,CA
中科院分区：
文献类型：
--
作者：
Jones,PJ;Pappu,AS;Illingworth,DR;Leitch,CA
为了评估两种能够立即识别人类胆固醇产生变化的技术的有效性，对 5 名健康个体在 48 小时内的血浆甲羟戊酸水平和氘吸收到血浆游离胆固醇的速率进行了比较。自由生活的受试者在研究前和研究期间自行选择一日三餐。在 t = 0 时，口服氧化氘。给药前和给药后每 4 小时采集一次血样。在每个时间点测定血浆中的总胆固醇和甲羟戊酸水平。相对于血浆水含量，血浆游离胆固醇的氘富集变化用于计算每个时间点的游离胆固醇分数合成率（FSR）。血浆总胆固醇水平保持恒定，而血浆甲羟戊酸和氘摄取方法均观察到显着的昼夜节律，最低点和峰值形成率分别出现在 14.00 至 16.00 小时和午夜左右。建议血浆甲羟戊酸水平和游离胆固醇氘摄取率技术都是短期测量人体胆固醇合成的合适技术。
To assess the validity of two techniques capable of identifying immediate changes in human cholesterol production, plasma mevalonic acid levels and the rate of uptake of deuterium into plasma free cholesterol were compared in 5 healthy individuals over 48 h. The free‐living subjects self‐selected three meals per day prior to and during study. At t = 0, deuterium oxide was administered orally. Blood samples were collected before and every 4 h after dosing. Total cholesterol and mevalonic acid levels were determined in plasma at each timepoint. Deuterium enrichment changes in plasma free cholesterol, relative to plasma water content, were used to calculate free cholesterol fractional synthetic rates (FSR) at each timepoint. Total plasma cholesterol levels remained constant, whereas significant circadian rhythmicity was observed in both plasma mevalonic acid and deuterium uptake methods, with nadir and peak formation rates indicated at 14.00 to 16.00 h and about midnight, respectively. It is suggested that plasma mevalonic acid levels and free cholesterol deuterium uptake rate techniques are both suitable techniques for short‐term measurement of human cholesterol synthesis.