ANTIGENIC POLYMORPHISM OF THE LAMB PROTEIN AMONG MEMBERS OF THE FAMILY ENTEROBACTERIACEAE
ANTIGENIC POLYMORPHISM OF THE LAMB PROTEIN AMONG MEMBERS OF THE FAMILY ENTEROBACTERIACEAE
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DOI：
10.1128/jb.163.1.106-110.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
DESAYMARD, C
中科院分区：
文献类型：
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作者：
BLOCH, MA;DESAYMARD, C
肠杆菌科的大多数成员显示具有与大肠杆菌K-12的LamB蛋白同源的麦芽糖诱导的外膜蛋白。这些蛋白质与针对大肠杆菌LamB蛋白的多克隆抗体反应。coli K-12。LamB蛋白的抗原结构在肠杆菌科成员中与针对大肠杆菌LamB蛋白的6种单克隆抗体进行比较。coli K-12。其中4个与位于膜外表面的表位反应，2个与位于膜内表面的表位反应。对于外部表位观察到很大程度的变异性。即使在单一种中，如E. coli中，存在一个重要的多态性。相比之下，内部表位更保守。
Most members of the family Enterobacteriaceae are shown to possess a maltose-inducible outer membrane protein homologous to the LamB protein of Escherichia coli K-12. These proteins react with polyclonal antibodies raised against the LamB protein of E. coli K-12. The antigenic structure of the LamB protein is compared in members of the family Enterobacteriaceae with 6 monoclonal antibodies raised against the LamB protein of E. coli K-12. Of them 4 reacted with epitopes located at the outer face of the membrane, and 2 reacted with epitopes located at the inner face of the membrane. A great degree of variability was observed for the external epitopes. Even in a single species, such as E. coli, an important polymorphism was present. In contrast, the internal epitopes were more conserved.