Nonexponential Kinetics of DNA Escape from α-Hemolysin Nanopores
Nonexponential Kinetics of DNA Escape from α-Hemolysin Nanopores
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DOI：
10.1529/biophysj.108.137760
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发表时间：
2008-12-01
影响因子：
3.4
通讯作者：
Marziali, Andre
中科院分区：
文献类型：
--
作者：
Wiggin, Matthew;Tropini, Carolina;Marziali, Andre
采用纳米尺度孔的DNA序列检测技术的吞吐量和分辨率取决于对纳米孔中DNA运动的准确动力学描述。我们首次对DNA从α-溶血素纳米孔中逃逸的动力学进行了详细的实验研究，并表明某些事件的逃逸时间异常长会导致非指数动力学。从第一次通过时间的分布，我们确定逃逸的能垒服从泊松分布，很可能是由于DNA和孔中氨基酸残基之间的随机弱结合事件。
Throughput and resolution of DNA sequence detection technologies employing nanometer scale pores hinge on accurate kinetic descriptions of DNA motion in nanopores. We present the first detailed experimental study of DNA escape kinetics from alpha-hemolysin nanopores and show that anomalously long escape times for some events result in nonexponential kinetics. From the distribution of first-passage times, we determine that the energy barrier to escape follows a Poisson-like distribution, most likely due to stochastic weak binding events between the DNA and amino acid residues in the pore.