ALTERATION OF NEURAL CELL-ADHESION MOLECULE (N-CAM) EXPRESSION AFTER NEURONAL CELL-TRANSFORMATION BY ROUS-SARCOMA VIRUS
ALTERATION OF NEURAL CELL-ADHESION MOLECULE (N-CAM) EXPRESSION AFTER NEURONAL CELL-TRANSFORMATION BY ROUS-SARCOMA VIRUS
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DOI：
10.1073/pnas.81.3.969
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
EDELMAN, GM
中科院分区：
文献类型：
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作者：
GREENBERG, ME;BRACKENBURY, R;EDELMAN, GM
通过免疫印迹、免疫荧光染色和使用粘附分子高度特异性抗体的体外细胞-细胞聚集测定来评估劳斯肉瘤病毒对大鼠神经细胞粘附分子 N-CAM 的转化效果。研究发现，在感染了对转化温度敏感的突变型劳斯肉瘤病毒的几种大鼠小脑细胞系中，N-CAM 的表达具有温度依赖性。在不允许的温度下，这些细胞显示出大量的 N-CAM，并通过 N-CAM 介导的机制快速聚集。当细胞系在允许的温度下生长时，它们发生形态转变，含有低得多的 N-CAM 并且聚集不良。在培养的原代大鼠小脑细胞和化学转化的神经元细胞系中均未观察到 N-CAM 表达的类似温度依赖性。在所有细胞系中，N-CAM 均以成体形式出现；迄今为止，已经在正常胚胎组织和成人大脑的一些区域中观察到了胚胎形式。显然，N-CAM 在细胞表面的分布可以通过转化来调节，对细胞间粘附有明显的影响。
The effect of transformation by Rous sarcoma virus on the [rat] neural cell adhesion molecule N-CAM was assessed by immunoblotting, immunofluorescence staining and an in vitro cell-cell aggregation assay using highly specific antibodies to the adhesion molecule. Expression of N-CAM was found to be temperature dependent in several rat cerebellar cell lines infected with a mutant Rous sarcoma virus that is temperature sensitive for transformation. At the nonpermissive temperature, these cells displayed significant quantities of N-CAM and aggregated rapidly by an N-CAM-mediated mechanism. When the cell lines were grown at the permissive temperature, they were morphologically transformed, contained much lower amounts of N-CAM and aggregated poorly. A similar temperature dependence of N-CAM expression was not observed in cultured primary rat cerebellar cells nor in a chemically transformed neuronal cell line. In all of the cell lines, N-CAM occurred in the adult forms; the embryonic form has so far been observed in normal embryonic tissues and a few regions of the adult brain. Evidently, N-CAM prevalence at the cell surface can be modulated by transformation with clear-cut effects on cell-cell adhesion.