Seasonality and Locality Affect the Diversity of Anopheles gambiae and Anopheles coluzzii Midgut Microbiota from Ghana.
Seasonality and Locality Affect the Diversity of Anopheles gambiae and Anopheles coluzzii Midgut Microbiota from Ghana.
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DOI：
10.1371/journal.pone.0157529
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wilson MD
中科院分区：
文献类型：
--
作者：
Akorli J;Gendrin M;Pels NA;Yeboah-Manu D;Christophides GK;Wilson MD
共生细菌在疾病媒介的发展和能力方面具有重要意义。在按蚊中，中肠微生物群的组成在很大程度上受到幼虫繁殖地点的影响，但目前尚不清楚形成这种组成的确切因素。在这里，我们研究了接近城市地区和季节是否对非洲两种主要疟疾媒介的中肠微生物群落产生影响。coluzzii和安氏隐翅虫An.冈比亚。在旱季和雨季期间，在加纳的两个地区，从选定的栖息地收集幼虫和蛹。从这些集合中出现的成年人的中肠微生物群通过16 S核糖体DNA的454焦磷酸测序来确定。我们发现，在这两种蚊子，希瓦氏菌科构成的平均54%和73%的中肠微生物群从每个网站在旱季和雨季，分别。在旱季，22/30个样品中发现了相对较低的丰度低于1%的肠杆菌科，在雨季，25/38个样品中发现了相对较低的丰度。我们的数据表明，季节性和局部性显著影响微生物群的多样性和细菌家族的相对丰度，并对旱季和城市周边环境产生积极影响。
Symbiotic bacteria can have important implications in the development and competence of disease vectors. In Anopheles mosquitoes, the composition of the midgut microbiota is largely influenced by the larval breeding site, but the exact factors shaping this composition are currently unknown. Here, we examined whether the proximity to urban areas and seasons have an impact on the midgut microbial community of the two major malaria vectors in Africa, An. coluzzii and An. gambiae. Larvae and pupae were collected from selected habitats in two districts of Ghana during the dry and rainy season periods. The midgut microbiota of adults that emerged from these collections was determined by 454-pyrosequencing of the 16S ribosomal DNA. We show that in both mosquito species, Shewanellaceae constituted on average of 54% and 73% of the midgut microbiota from each site in the dry and rainy season, respectively. Enterobacteriaceae was found in comparatively low abundance below 1% in 22/30 samples in the dry season, and in 25/38 samples in the rainy season. Our data indicate that seasonality and locality significantly affect both the diversity of microbiota and the relative abundance of bacterial families with a positive impact of dry season and peri-urban settings.