Intracellular proliferation of clover yellow vein virus is unaffected by the recessive resistance gene cyv1 of Pisum sativum
Intracellular proliferation of clover yellow vein virus is unaffected by the recessive resistance gene cyv1 of Pisum sativum
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DOI：
10.1111/1348-0421.12755
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发表时间：
2020-01
影响因子：
2.6
通讯作者：
Yosuke Taninaka;K. Nakahara;Yuka Hagiwara-Komoda
中科院分区：
文献类型：
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作者：
Yosuke Taninaka;K. Nakahara;Yuka Hagiwara-Komoda
豌豆cyv1基因是一个尚未鉴定的抑制三叶草黄脉病毒(ClYVV)侵染的隐性抗性基因。以前的研究证实，在携带Cyv1的豌豆植株中，ClYVV的细胞间移动受到抑制；然而，Cyv1对病毒复制的影响尚不清楚。在本研究中，我们开发了一种新的豌豆原生质体转染法，以在单细胞水平上研究ClYVV的繁殖。利用这种方法，我们发现在豌豆原生质体中，ClYVV在敏感的和携带Cyv1的原生质体中积累到相似的水平。因此，Cyv1介导的抗性不会抑制细胞内的ClYVV复制。
The pea cyv1 gene is a yet‐to‐be‐identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell‐to‐cell movement of ClYVV is inhibited in cyv1‐carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this study, we developed a new pea protoplast transfection method to investigate ClYVV propagation at the single‐cell level. Using this method, we revealed that ClYVV accumulates to similar levels in both ClYVV‐susceptible and cyv1‐carrying pea protoplasts. Thus, the cyv1‐mediated resistance would not suppress intracellular ClYVV replication.