Silver-staining patterns of mammalian epididymal spermatozoa.
Silver-staining patterns of mammalian epididymal spermatozoa.
复制标题
哺乳动物附睾精子的银染图案。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
T. Hsu
中科院分区：
文献类型：
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作者：
F. Elder;T. Hsu
用银硝酸盐对12种哺乳动物附睾精子进行染色，并在光学显微镜下观察。银可区分精子的许多大体形态特征，包括顶体、顶体下区域、穿孔器、顶体后鞘、颈部、中段核心的致密外纤维、环带、主片和终片。精子的银染模式揭示了种属特异性和品系特异性的差异，特别是精子头部。银染色的生化基础可能部分是由于存在富含巯基和二硫化物的蛋白质;然而，不能完全用这些部分的存在来解释。使用硝酸银染色获得的细节，加上该过程的简便和快速，应该对生物和医学研究的许多领域的工作人员有用。
Epididymal spermatozoa from 12 species of mammals were stained using silver nitrate and examined with the light microscope. Silver nitrate differentiates many of the gross morphological features of spermatozoa, including the acrosome, subacrosomal region, perforatorium, postacrosomal sheath, neck, dense outer fibers of the core of the midpiece, annulus, principal piece, and end piece. Silver-staining patterns of spermatozoa reveal both species-specific and strain-specific differences, particularly of the sperm head. The biochemical basis of silver staining may be due in part to the presence of sulfhydryl- and disulfide-rich proteins; however, it cannot be explained entirely by the presence of these moieties. The detail obtained using silver nitrate staining, coupled with the ease and rapidity of the procedure, should be useful to workers in many areas of biological and medical research.