Overgrowth of mice generated from postovulatory aged oocyte spindles.
Overgrowth of mice generated from postovulatory aged oocyte spindles.
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排卵后老化卵母细胞纺锤体产生的小鼠过度生长。
DOI：
10.1096/fba.2019-00005
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发表时间：
2019
影响因子：
2.7
通讯作者：
Kawahara M.
中科院分区：
文献类型：
--
作者：
Shiina K;Komatsu M;Yokoi F;Bai H;Takahashi T;Kawahara M.
卵母细胞纺锤体转移（OST）是一种用于哺乳动物的有效生殖技术，它使第二次减数分裂（MII）中期变质的卵母细胞中的纺锤体作为产生后代的遗传物质。然而，是否使用发育恶化的MII卵母细胞通过OST实现出生后生长仍不清楚。在人绒毛膜促性腺激素给药后16小时，从输卵管取出后立即剥离的MII卵母细胞（0 h卵母细胞）用于体外受精（IVF）作为对照。对于体外受精，使用排卵后老化的卵母细胞，0小时卵母细胞进一步孵育12小时和24小时（12小时和24小时卵母细胞）。这些小鼠卵母细胞被用作评估由发育恶化的卵母细胞通过OST产生的个体出生后生长的模型。12 h和24 h卵母细胞纺锤体的胚胎在新生儿阶段的发育速度与未处理的对照组一样高。然而，与对照组和12 h卵母细胞纺锤体来源的小鼠相比，24 h卵母细胞纺锤体来源的小鼠表现出更重的体重和更高的饲料消耗。我们的研究结果证明了OST作为一种有效的生殖技术的可行性，以及它在哺乳动物生殖中使用过老的排卵后卵母细胞的局限性。
Oocyte spindle transfer (OST) is a potent reproductive technology used for mammals that enables the spindle in a deteriorated oocyte at the metaphase of the second meiotic division (MII) to serve as the genetic material for producing descendants. However, whether postnatal growth is achieved via OST using developmentally deteriorated MII oocytes remains unclear. At 16 h after human chorionic gonadotropin administration, denuded MII oocytes immediately after retrieval from oviducts (0 h‐oocytes) were used for in vitro fertilization (IVF) as controls. For IVF using postovulatory‐aged oocytes, the 0 h‐oocytes were further incubated for 12 h and 24 h (12 h‐ and 24 h‐oocytes). These mouse oocytes served as a model for assessing the postnatal growth of individuals produced via OST from developmentally deteriorated oocytes. The embryos from 12 h‐ and 24 h‐oocyte spindles exhibited high rates of development up to the neonatal stage as good as the non‐manipulated controls. However, the mice derived from the 24 h‐oocyte spindles displayed heavier body weights and greater feed consumption than both controls and mice derived from 12 h‐oocyte spindles. Our results demonstrate the feasibility of OST as a potent reproductive technology and its limitation in the use of excessively aged postovulatory oocytes in mammalian reproduction.