Expression and regulation of adiponectin and receptor in human and rat placenta
Expression and regulation of adiponectin and receptor in human and rat placenta
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DOI：
10.1210/jc.2004-0930
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发表时间：
2005-07-01
影响因子：
5.8
通讯作者：
Diéguez, C
中科院分区：
文献类型：
--
作者：
Caminos, JE;Nogueiras, R;Diéguez, C
背景：脂联素是一种脂肪细胞激素，参与糖脂代谢。最近，这种蛋白质的两种受体，称为脂联素受体1，Adipo-R1和Adipo-R2基因已被克隆。目的：本研究的目的是检测脂联素及其受体是否在人和大鼠胎盘中表达，并评估胎龄和营养状况对这些因子的调节作用。我们的研究结果表明，脂联素和Adipo-R2定位在人类和大鼠胎盘。人脂联素和Adipo-R2存在于细胞滋养层和合胞体滋养层细胞中。然而，大鼠脂联素和Adipo-R2在妊娠期间改变其特异性细胞类型免疫染色。此外，胎盘脂联素而Adipo-R2具有相反的模式。我们还评估了妊娠期食物限制（30%）的影响，我们观察到脂联素mRNA水平在营养不良16天后下降。相反，胎盘Adipo-R2 mRNA不受营养不良的影响。最后，治疗与脂联素在妊娠期减少脂肪-R2，葡萄糖转运蛋白3，脂蛋白脂肪酶，和TGF-β mRNA的expression.Conclusion：两者合计，我们的研究结果表明，至少在啮齿动物，脂联素可能参与调节几个胎盘功能。
Context: Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. Recently, two receptors of this protein, called adiponectin receptor 1 (Adipo-R1) and Adipo-R2, have been cloned.Objective: The aim of this study was to examine whether adiponectin and its receptors are expressed in human and rat placentas and to evaluate the regulation of these factors by gestational age and nutritional status.Results: Our results demonstrate that adiponectin and Adipo-R2 are localized in both human and rat placentas. Human adiponectin and Adipo-R2 are presented in cytotrophoblast and syncytiotrophoblast cells. However, rat adiponectin and Adipo-R2 change their specific cell type immunostaining during gestation. Furthermore, placental adiponectin whereas Adipo-R2 has the contrary pattern. We also assessed the effect of food restriction ( 30%) during gestation, and we observed that adiponectin mRNA levels decrease after 16 d of undernutrition. In contrast, placental Adipo-R2 mRNA is unchanged by undernutrition. Finally, treatment with adiponectin during gestation decreases Adipo-R2, glucose transporter 3, lipoprotein lipase, and TGF-beta mRNA expression.Conclusion: Taken together, our results suggest that, at least in rodents, adiponectin may be involved in the regulation of several placental functions.