miR-338-5p Levels and Cigarette Smoking are Associated With Neuropathic Pain Severity in Individuals With Spinal Cord Injury: Preliminary Findings From a Genome-Wide microRNA Expression Profiling Screen
miR-338-5p Levels and Cigarette Smoking are Associated With Neuropathic Pain Severity in Individuals With Spinal Cord Injury: Preliminary Findings From a Genome-Wide microRNA Expression Profiling Screen
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DOI：
10.1016/j.apmr.2021.09.005
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发表时间：
2022-03-31
影响因子：
4.3
通讯作者：
Morse, Leslie R.
中科院分区：
文献类型：
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作者：
Kowalski, Jesse L.;Nguyen, Nguyen;Morse, Leslie R.
目的：探讨脊髓损伤后神经病理性疼痛的相关microRNA生物标志物及临床因素。设计：对从正在进行的临床研究中收集的基线数据进行横断面、二级分析。使用全基因组microRNA筛选方法，我们研究了43名参与正在进行的临床研究的脊髓损伤成人血清中microRNA的差异表达。最小二乘回归用于确定microRNA表达、临床因素和神经性疼痛严重程度之间的关联。背景：社区居住的脊髓损伤患者。nbsp;你好参加人员：参与者（N=43）至少18岁，脊髓损伤，28人报告神经性疼痛，15人报告无神经性疼痛。干预措施：不适用。主要结果指标：使用国际脊髓损伤疼痛基本数据集评估疼痛的存在、类型和强度。从血液样品定量血清microRNA标准化深度测序计数。通过问卷调查收集参与者的人口统计学因素、损伤特征、药物使用和健康习惯。nbsp;你好结果如下：miR-338- 5 p表达和吸烟史与神经病理性疼痛严重程度相关，并解释了37%的变异（R-2=0.37，F-2，F-18=5.31，P= 0.02），独立于其他临床因素。miR-338- 5 p水平与伤害性疼痛严重程度之间没有关联。nbsp;你好结论：我们的研究结果表明，miR-338- 5 p和吸烟可能都在脊髓损伤后神经病理性疼痛的发展或维持中发挥作用。虽然需要更多的工作来证实这些发现，但经验证的靶点分析表明miR-338- 5 p在调节神经炎症和神经元凋亡中具有神经保护作用，并且其下调可能导致导致脊髓损伤后神经性疼痛的适应不良的神经可塑性机制。(C)2021年美国康复医学大会。由爱思唯尔公司出版All rights reserved.
Objective: To identify microRNA biomarkers and clinical factors associated with neuropathic pain after spinal cord injury.& nbsp;Design: Cross-sectional, secondary analysis of baseline data collected from ongoing clinical studies. Using a genome-wide microRNA screening approach, we studied differential microRNA expression in serum from 43 adults with spinal cord injury enrolled in ongoing clinical studies. Least squares regression was used to identify associations between microRNA expression, clinical factors, and neuropathic pain severity.& nbsp;Setting: Community-dwelling individuals with spinal cord injury.& nbsp;Participants: Participants (N=43) were at least 18 years old with spinal cord injury, with 28 reporting neuropathic pain and 15 reporting no neuropathic pain.& nbsp;Interventions: Not applicable.& nbsp;Main Outcome Measures: Pain presence, type, and intensity were assessed with the International Spinal Cord Injury Pain Basic Data Set. Serum microRNA normalized deep sequencing counts were quantified from blood samples. Participant demographic factors, injury characteristics, medication use, and health habits were collected via questionnaire.& nbsp;Results: miR-338-5p expression and history of cigarette smoking were associated with and explained 37% of the variance in neuropathic pain severity (R-2=0.37, F-2,F-18=5.31, P=.02) independent of other clinical factors. No association was identified between miR-338-5p levels and nociceptive pain severity.& nbsp;Conclusions: Our findings suggest that miR-338-5p and cigarette smoking may both play a role in the development or maintenance of neuropathic pain after spinal cord injury. While additional work is needed to confirm these findings, validated target analysis suggests a neuroprotective role of miR-338-5p in modulating neuroinflammation and neuronal apoptosis and that its downregulation may result in maladaptive neuroplastic mechanisms contributing to neuropathic pain after spinal cord injury. (C) 2021 The American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.