Synthesis of hybrid sol-gel materials and their biological evaluation with human mesenchymal stem cells
Synthesis of hybrid sol-gel materials and their biological evaluation with human mesenchymal stem cells
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DOI：
10.1007/s10856-013-4900-y
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发表时间：
2013-06-01
影响因子：
3.7
通讯作者：
Gurruchaga, M.
中科院分区：
文献类型：
--
作者：
Hernandez-Escolano, M.;Juan-Diaz, M. J.;Gurruchaga, M.
生物材料的表面工程可以促进种植体的骨结合。在这项工作中，两种类型的混合溶胶-凝胶材料的开发，以刺激细胞附着，增殖和分化的成骨细胞。一种是由乙烯基三乙氧基硅烷（VTES）和正硅酸乙酯（TEOS）以不同的摩尔比合成，而另一种是由VTES和羟基磷灰石颗粒（HAp）。采用核磁共振、傅里叶变换红外光谱和接触角测量等方法对杂化材料进行了系统的研究。涂层的生物相容性和骨诱导性通过使用MTT测定测量间充质干细胞增殖和通过定量富钙沉积物分析矿化细胞外基质产生来评价。结果突出了这些涂层在通过改变VTES：TEOS前体的摩尔比获得不同性质方面的通用性。因此，矿化刺激TEOS含量的增加，而除了HAp提高细胞增殖，但恶化矿化。
Surface engineering of biomaterials could promote the osseointegration of implants. In this work, two types of hybrid sol-gel materials were developed to stimulate cell attachment, proliferation and differentiation of osteogenic cells. One type was synthesised from vinyl triethoxysilane (VTES) and tetraethyl-orthosilicate (TEOS) at different molar ratios, while the other from VTES and hydroxyapatite particles (HAp). Hybrid materials were systematically investigated using nuclear magnetic resonance, Fourier transform infrared spectroscopy and contact angle metrology. The biocompatibility and osseoinduction of the coatings were evaluated by measuring mesenchymal stem cell proliferation using MTT assays and analysing the mineralised extracellular matrix production by quantifying calcium-rich deposits. The results highlighted the versatility of these coatings in obtaining different properties by changing the molar ratio of the VTES:TEOS precursors. Thus, mineralisation was stimulated by increasing TEOS content, while the addition of HAp improved cell proliferation but worsened mineralisation.