2 OVERLAPPING GENES IN BOVINE MITOCHONDRIAL-DNA ENCODE MEMBRANE-COMPONENTS OF ATP SYNTHASE
2 OVERLAPPING GENES IN BOVINE MITOCHONDRIAL-DNA ENCODE MEMBRANE-COMPONENTS OF ATP SYNTHASE
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DOI：
10.1002/j.1460-2075.1986.tb04456.x
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发表时间：
1986-08-01
期刊：
影响因子：
11.4
通讯作者：
WALKER, JE
中科院分区：
文献类型：
--
作者：
FEARNLEY, IM;WALKER, JE
用氯仿：甲醇混合物从牛线粒体中提取约13种蛋白质，将两种疏水蛋白纯化至均匀性。序列分析表明，较小的是一个由66个氨基酸组成的蛋白质，是线粒体基因A6L的产物。较大的一种是ATP -6，由226个氨基酸组成，是ATP合成酶的一种膜成分，也编码于线粒体DNA中。测定的蛋白序列表明，这两个蛋白的基因重叠了40个碱基，并表明第二个基因ATPase-6的翻译是在A6L的编码区开始的。A6L和ATP -6蛋白也从ATP合成酶复合体中分离出来，因此似乎是该酶的真正组成部分。A6L的功能未知。然而，较弱的结构同源性表明其功能与酵母线粒体蛋白aapI相似，aapI是真菌ATP合成酶复合物组装所必需的。atp -6与亚基α的同源性。大肠杆菌ATP合酶复合体的研究表明，ATP -6蛋白在线粒体复合体中的作用与其细菌的对应物相似，对活性质子通道的形成至关重要。
Two hydrophobic proteins have been purified to homogeneity from a mixture of about 13 proteins that are extracted from bovine mitochondria with a chloroform:methanol mixture. Sequence analysis shows that the smaller is a protein of 66 amino acids and is the product of a mitochondrial gene, A6L. The larger, a protein of 226 amino acids, is ATPase-6, a membrane component of ATP synthase, also encoded in mitochondrial DNA. The protein sequences determined establish that the genes for the two proteins overlap by 40 bases and indicate that translation of the second gene, ATPase-6, is initiated within the coding region of A6L. The A6L and the ATPase-6 proteins have also been isolated from the ATP synthase complex and so appear to be bona fide components of the enzyme. The function of A6L is unknown. However, weak structural homology suggests a functional similarity to the yeast mitochondrial protein, aapI, which is required for assembly of the fungal ATP synthase complex. Homologies between ATPase-6 and subunit .alpha. of the Escherichia coli ATP synthase complex indicate that the ATPase-6 protein has a similar role in the mitochondrial complex to its bacterial counterpart, being essential for the formation of an active proton channel.