A method for the synthesis of isomerically pure saturated mixed-chain phosphatidylcholines.
A method for the synthesis of isomerically pure saturated mixed-chain phosphatidylcholines.
复制标题
一种合成异构纯饱和混链磷脂酰胆碱的方法。
DOI：
10.1016/0003-2697(81)90049-x
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发表时间：
1981
影响因子：
2.9
通讯作者：
Huang,C
中科院分区：
文献类型：
--
作者：
Mason,JT;Broccoli,AV;Huang,C
描述了一种高纯度（通常为 99 mol%）饱和混合链磷脂酰胆碱的部分合成方法。该程序旨在消除对称链磷脂酰胆碱和所需产物的混合链异构体（先前技术引入的两种主要杂质）对混合链产物的污染。这种高纯度是通过采用专为饱和对称磷脂酰胆碱中的 C-2 脂肪酰基部分进行完全酶水解而设计的方法以及使用催化剂 4-吡咯烷基吡啶酰化溶血磷脂酰胆碱的新技术而获得的。通过几种饱和混合链磷脂酰胆碱的合成说明了这种新方法的多功能性。
A method is described for the partial synthesis of saturated mixed-chain phosphatidylcholines of a high degree (typically 99 mol%) of purity. This procedure has been designed to eliminate the contamination of the mixed-chain product by symmetric chain phosphatidylcholine and the mixed-chain isomer of the desired product, the two principal impurities introduced by previous techniques. This high degree of purity is obtained by employing a method designed for the complete enzymatic hydrolysis of the C-2 fatty acyl moiety in saturated symmetric phosphatidylcholines and a new technique for the acylation of lysophosphatidylcholines employing the catalyst 4-pyrrolidinopyridine. The versatility of this new procedure is illustrated with the synthesis of several saturated mixed-chain phosphatidylcholines.