Discovery and Characterization of a Bilirubin Inducible Green Fluorescent Protein From the Moray Eel Gymnothorax zonipectis
Discovery and Characterization of a Bilirubin Inducible Green Fluorescent Protein From the Moray Eel Gymnothorax zonipectis
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DOI：
10.3389/fmars.2021.678571
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发表时间：
2021-09
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影响因子：
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通讯作者：
Andrew M. Guarnaccia;Sara R Krivoshik;J. Sparks;D. F. Gruber;Jean P. Gaffney
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作者：
Andrew M. Guarnaccia;Sara R Krivoshik;J. Sparks;D. F. Gruber;Jean P. Gaffney
自从在华盛顿普吉湾海岸首次发现维多利亚水藻的绿色荧光以来，生物荧光海洋生物已经在地球仪上被发现。生物荧光颜色的多样性以及表现出这种荧光的生物体的可变性令人震惊。海洋生物中的生物荧光机制也各不相同。为了发出荧光，一些生物体使用荧光蛋白，而另一些则使用小分子。在鳗鱼中，绿色生物荧光首先在日本鳗鲡中鉴定。A.日本的发现是由脂肪酸结合蛋白（UnaG）引起的，其荧光是由胆红素的加入引起的。这类蛋白质的成员后来在Kaupichthys鳗鱼中发现（Chlopsid FP I和Chlopsid FP II）。在这里，我们报告了从海鳗裸胸带中发现和表征这类绿色荧光脂肪酸结合蛋白的第一个成员。该蛋白质GymFP为15.6 kDa，加入胆红素后在496 nm处激发荧光，在532 nm处发射最大值。GymFP与UnaG的同源性为61%，与Chlopsid FP I的同源性为47%。在这里，我们报告从头转录组组装，蛋白质表达和荧光光谱表征GymFP。这些发现将荧光脂肪酸结合蛋白扩展到真鳗鱼（鳗鲡目）的第三个家族。
Since the initial discovery of Aqueoria victoria’s green fluorescence off the coast of Washington’s Puget Sound, biofluorescent marine organisms have been found across the globe. The variety of colors of biofluorescence as well as the variability in the organisms that exhibit this fluorescence is astounding. The mechanisms of biofluorescence in marine organisms are also variable. To fluoresce, some organisms use fluorescent proteins, while others use small molecules. In eels, green biofluorescence was first identified in Anguilla japonica. The green fluorescence in A. japonica was discovered to be caused by a fatty acid binding protein (UnaG) whose fluorescence is induced by the addition of bilirubin. Members of this class of proteins were later discovered in Kaupichthys eels (Chlopsid FP I and Chlopsid FP II). Here, we report the discovery and characterization of the first member of this class of green fluorescent fatty acid binding proteins from the moray eel Gymnothorax zonipectis. This protein, GymFP, is 15.6 kDa with a fluorescence excitation at 496 nm and an emission maximum at 532 nm upon addition of bilirubin. GymFP is 61% homologous to UnaG and 47% homologous to Chlopsid FP I. Here, we report de novo transcriptome assembly, protein expression, and fluorescence spectroscopic characterization of GymFP. These findings extend the fluorescent fatty acid binding proteins into a third family of true eels (Anguilliformes).