THE INTERNATIONAL STANDARDS FOR INTERLEUKIN-1-ALPHA AND INTERLEUKIN-1-BETA - EVALUATION IN AN INTERNATIONAL COLLABORATIVE STUDY
THE INTERNATIONAL STANDARDS FOR INTERLEUKIN-1-ALPHA AND INTERLEUKIN-1-BETA - EVALUATION IN AN INTERNATIONAL COLLABORATIVE STUDY
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DOI：
10.1016/0022-1759(91)90286-o
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发表时间：
1991-08-28
影响因子：
2.2
通讯作者：
DAS, REG
中科院分区：
文献类型：
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作者：
POOLE, S;DAS, REG
由6个国家的13个实验室评价了白细胞介素-1-α（IL-1-α）的3种安瓿制剂和白细胞介素-1-β（IL-1-β）的3种安瓿制剂作为这些材料的国际标准品的适用性。 在体外和体内生物测定，放射受体测定和免疫测定的制剂进行了测定。 根据本文报告的结果，经研究参与者同意并经世界卫生组织（WHO）生物标准化专家委员会（ECBS）授权，将其中一种IL-1-α制剂和一种IL-1-β制剂确立为这些材料的国际标准品。 此外，由于IL-1-α和IL-1-β的相对活性取决于所用的测定系统，因此决定应参考IL-1-α活性或IL-1-β活性的单位，而不是IL-1活性。
Three ampouled preparations of interleukin-1-alpha (IL-1-alpha) and three ampouled preparations of interleukin-1-beta (IL-1-beta) were evaluated by 13 laboratories in six countries for their suitability to serve as international standards for these materials. The preparations were assayed in in vitro and in vivo bioassays, radioreceptor assays and immunoassays. On the basis of the results reported here, with the agreement of participants in the study and with the authorization of the Expert Committee on Biological Standardization (ECBS) of the World Health Organization (WHO), one of the preparations of IL-1-alpha and one of the preparations of IL-1-beta were established as international standards for these materials. Further, since the relative activities of IL-1-alpha and IL-1-beta were dependent upon the assay systems used, it was decided that reference should be made to units of IL-1-alpha activity or IL-1-beta activity rather than to IL-1 activity.