Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver
Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver
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DOI：
10.1016/j.bbrc.2011.03.133
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发表时间：
2011-04-29
影响因子：
3.1
通讯作者：
Nishina, Hiroshi
中科院分区：
文献类型：
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作者：
Miyamura, Norio;Nakamura, Takashi;Nishina, Hiroshi
部分肝切除术 (PH) 后，再生肝脏会积累未知的脂质种类。在这里，我们通过薄层色谱 (TLC)、成像质谱 (IMS) 和实时 RT-PCR 分析了 PH 后小鼠肝脏和脂肪组织中的脂质。在肝脏中，IMS 显示单个 TLC 带包含主要的 19 种 TG 种类。同样，IMS 显示单个磷脂 TLC 条带主要有 13 种。在脂肪组织中，PH 诱导调节脂质代谢的基因表达发生变化。最后，磷脂酰胆碱种类的 IMS 显示了类似于肝分区的小叶分布梯度。因此，IMS 是一种用于高分辨率分析脂质种类的新颖且强大的工具。 (C) 2011 Elsevier Inc. 保留所有权利。
After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution. (C) 2011 Elsevier Inc. All rights reserved.