Differential skeletal response in adult and aged rats to independent and combinatorial stimulation with pulsed electromagnetic fields and mechanical vibration
Differential skeletal response in adult and aged rats to independent and combinatorial stimulation with pulsed electromagnetic fields and mechanical vibration
复制标题
成年和老年大鼠对脉冲电磁场和机械振动的独立和组合刺激的差异骨骼反应
DOI：
10.1096/fj.201902779r
复制
发表时间：
2019-12-29
期刊：
影响因子：
4.8
通讯作者：
Jing, Da
中科院分区：
文献类型：
--
作者：
Cai, Jing;Shao, Xi;Jing, Da
脉冲电磁场（PEMFs）和全身振动（WBV）被证明可以部分保留后肢卸载和卵巢切除动物的骨量/强度。然而，PEMF或WBV的潜在年龄依赖性骨骼反应尚未得到充分研究。此外，耦合的“力-电-磁”信号是否可以诱导比单一刺激更大的成骨潜力仍然是未知的。在此，将5月龄或20月龄的大鼠分配到对照组、PEMF组、WBV组和PEMF + WBV组。治疗8周后，单次PEMF/WBV增强了5月龄大鼠的骨量、强度和骨密度，但在20月龄大鼠中没有。PEMF + WBV较单纯PEMF/WBV能显著提高幼龄大鼠的骨量、骨质量和骨强度。PEMF + WBV也可通过提高成骨细胞和骨细胞活性抑制老年大鼠骨丢失，但对骨吸收无影响。PEMF + WBV可上调衰老骨骼中各种经典Wnt配体及其下游分子（p-GSK-3 beta和beta-catenin）的表达，但对非经典Wnt 5a的表达无影响，提示经典Wnt信号通路可能参与PEMF + WBV的骨修复。这项研究不仅揭示了老年人骨骼对单一PEMF/WBV的反应性相对于年轻人骨骼要弱得多，而且还提出了一种基于PEMF + WBV组合治疗的新的非侵入性方法，用于改善骨骼健康和保持骨数量/质量（特别是对于与年龄相关的骨质疏松症），具有更强的合成代谢作用。
Pulsed electromagnetic fields (PEMFs) and whole-body vibration (WBV) are proved to partially preserve bone mass/strength in hindlimb-unloaded and ovariectomized animals. However, the potential age-dependent skeletal response to either PEMF or WBV has not been fully investigated. Moreover, whether the coupled "mechano-electro-magnetic" signals can induce greater osteogenic potential than single stimulation remains unknown. Herein, 5-month-old or 20-month-old rats were assigned to the Control, PEMF, WBV, and PEMF + WBV groups. After 8-week treatment, single PEMF/WBV enhanced bone mass, strength, and anabolism in 5-month-old rats, but not in 20-month-old rats. PEMF + WBV induced greater increase of bone quantity, quality, and anabolism than single PEMF/WBV in young adult rats. PEMF + WBV also inhibited bone loss in elderly rats by primarily improving osteoblast and osteocyte activity, but had no effects on bone resorption. PEMF + WBV upregulated the expression of various canonical Wnt ligands and downstream molecules (p-GSK-3 beta and beta-catenin), but had no impacts on noncanonical Wnt5a expression in aged skeleton, revealing the potential involvement of canonical Wnt signaling in bone anabolism of PEMF + WBV. This study not only reveals much weaker responsiveness of aged skeleton to single PEMF/WBV relative to young adult skeleton, but also presents a novel noninvasive approach based on combinatorial treatment with PEMF + WBV for improving bone health and preserving bone quantity/quality (especially for age-related osteoporosis) with stronger anabolic effects.