Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources.
Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources.
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DOI：
10.4317/jced.53957
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发表时间：
2018-01
影响因子：
--
通讯作者：
Mohamed A
中科院分区：
文献类型：
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作者：
Aboushady IM;Salem ZA;Sabry D;Mohamed A
间充质干细胞（MSC）可以再生缺失的组织和治疗疾病。因此，本研究旨在比较骨髓间充质干细胞（BMSCs）、牙龈间充质干细胞（GMSCs）和下颌下间充质干细胞（SMSCs）的增殖率和成骨分化潜能。从大鼠骨髓、牙龈和颌下腺分离培养间充质干细胞。MTT法检测细胞增殖能力;通过Alzarin红染色评估成骨分化，并对Runx-2和MMP-13进行定量RT-PCR。与GMSC和SMSC相比，在BMSC中估计最高的显著增殖（p值< 0.01）。所有研究的细胞类型在分化的第3代形成矿化结节，用茜素红染色。然而，与GMSC和SMSC相比，BMSC似乎产生最高水平的矿化。RT-PCR结果显示，骨髓间充质干细胞中Runx-2和MMP-13 mRNA的表达明显高于骨髓间充质干细胞和平滑肌细胞（p < 0.01）。BMSC显示最大的成骨结果，其次是GMSC，最后是SGSC。因此，可以推荐GMSCs作为BMSCs之后的第二选择，当需要骨组织重建时。 关键词：间充质干细胞，成骨分化，Runx-2，MMP-13。
Mesenchymal stem cells (MSCs) can regenerate missing tissues and treat diseases. Hence, the current work aimed to compare the proliferation rate and the osteogenic differentiation potential of bone marrow MSCs (BMSCs), gingival MSCs (GMSCs) and submandibular MSCs (SMSCs). MSCs derived from bone marrow, gingiva and submandibular salivary gland were isolated and cultured from rats. The proliferation capacity was judged by MTT proliferation Assay. Osteogenic differentiation was assessed by Alzarin red stain and quantitative RT-PCR was performed for Runx-2 and MMP-13. The highest significant proliferation was estimated in the BMSCs compared to GMSCs and SMSCs (p-value was < 0.01). All studied cell types formed mineralized nodules as stained with Alizarin Red stain at the 3rd passage of differentiation. However, BMSCs seemed to generate the highest level of mineralization compared to GMSCs and SMSCs. RT-PCR revealed that the expression of Runx-2 and MMP-13 mRNAs was significantly increased in the BMSCs compared to GMSCs and SMSCs (p-value was < 0.01). BMSCs displayed maximum osteogenesis results followed by the GMSCs and lastly by the SGSCs. Thus, it could be recommended that GMSCs can be used as a second choice after BMSCs when bone tissue reconstruction is needed. Key words:Mesenchymal stem cells, osteogenic differentiation, Runx-2, MMP-13.