Cannabinoids increase conditioned ultrasonic vocalisations and cat odour avoidance in rats: Strain differences in drug-induced anxiety
Cannabinoids increase conditioned ultrasonic vocalisations and cat odour avoidance in rats: Strain differences in drug-induced anxiety
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DOI：
10.1016/j.lfs.2010.09.018
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发表时间：
2010-10-23
期刊：
影响因子：
6.1
通讯作者：
McGregor, Lain S.
中科院分区：
文献类型：
--
作者：
Arnold, Jonathon C.;Dielenberg, Robert A.;McGregor, Lain S.
目的：遗传倾向调节大麻的精神作用。与Lewis品系大鼠相比，大麻素对Wistar中负责焦虑的大脑区域有更大的影响。在这里，我们的目的是表明这与大麻素诱导的焦虑相关行为的菌株差异相关。主要方法：Lewis和Wistar大鼠在条件超声发声（USV）模型、猫臭躲避模型和开放区域躲避模型中分别给药合成大麻素受体激动剂CP 55,940（10、25和50 mu g/kg）。主要发现：动物们被放置在一个房间里，在这个房间里它们之前接受过足震。Wistar大鼠而非Lewis大鼠再次暴露在所有CP 55,940剂量的影响下，其释放的usv明显高于载具处理大鼠。在猫气味回避模型中，老鼠暴露在猫气味中，并有机会躲在一个小盒子里。在Wistar大鼠而非Lewis大鼠中，50 pg/kg的CP 55,940放大了猫气味引起的隐藏行为。动物也在开放区域躲避模型中进行了测试，该模型发生在与掠食性躲避模型相同的场所，但没有猫的气味。在Wistar大鼠中，25和50 μ g/kg的CP 55,940增加了对开放空间的回避，但在Lewis大鼠中没有。意义：CP 55,940增加了Wistar大鼠而非Lewis大鼠的焦虑相关行为，为解剖大麻素诱导焦虑的遗传基础提供了模型。我们首次表明大麻素放大了条件usv和猫气味回避行为，这取决于被测试的菌株。英国皇家版权所有(C) 2010出版的爱思唯尔公司。版权所有。
Aims: Genetic disposition modulates the psychoactive effects of cannabis. Cannabinoids have a greater impact on brain regions that subserve anxiety in Wistar compared to Lewis strain rats. Here we aim to show that this correlates with strain differences in cannabinoid-induced anxiety-related behaviour.Main methods: Lewis and Wistar rats were administered vehicle or the synthetic cannabinoid receptor agonist, CP 55,940 (10, 25 and 50 mu g/kg) before testing in the conditioned ultrasonic vocalization (USV), cat odour avoidance or open area avoidance models.Key findings: Animals were placed in a chamber in which they had previously received footshock. Wistar but not Lewis rats re-exposed under the influence of all CP 55,940 doses emitted significantly more USVs than vehicle-treated rats. In the cat odour avoidance model, rats were exposed to cat odour and given the opportunity to hide in a small box. In Wistar but not Lewis rats, 50 pg/kg of CP 55,940 magnified hiding behaviour promoted by cat odour exposure. Animals were also tested in the open area avoidance model which occurred in the same arena as the predatory avoidance model but without cat odour. In Wistar, but not Lewis rats, 25 and 50 mu g/kg of CP 55,940 increased the avoidance of the open space.Significance: CP 55,940 increased anxiety-related behaviour in Wistar rats but not Lewis rats providing a model to dissect the genetic basis of cannabinoid-induced anxiety. We show for the first time that cannabinoids magnify conditioned USVs and cat odour avoidance behaviour dependent on the strain being tested. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.