Prediction of secondary structural elements in glycerol-3-phosphate dehydrogenase by comparison with other dehydrogenases.
Prediction of secondary structural elements in glycerol-3-phosphate dehydrogenase by comparison with other dehydrogenases.
复制标题
通过与其他脱氢酶比较来预测 3-磷酸甘油脱氢酶的二级结构元件。
DOI：
10.1111/j.1432-1033.1980.tb04798.x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Rossmann,MG
中科院分区：
文献类型：
--
作者：
Otto,J;Argos,P;Rossmann,MG
从其氨基酸序列预测了甘油-3-磷酸脱氢酶的二级结构。在多肽的前半部分内的螺旋和片层的模式以及特异性标记残基与其它内切酶中NAD结合结构域的性质一致。序列的后半部分显示与甘油醛-3-磷酸脱氢酶的催化结构域的相似性。由此产生的甘油-3-磷酸脱氢酶的双结构域结构为烟酰胺环和L-甘油-3-磷酸底物的B特异性提供了正确的环境。
The secondary structure of glycerol‐3‐phosphate dehydrogenase was predicted from its amino acid sequence. The pattern of helices and sheets within the first half of the polypeptide as well as specific marker residues were consistent with the properties of the NAD binding domain in other dehydrogenases. The second half of the sequence shows similarities with the catalytic domain of glyceraldehyde‐3‐phosphate dehydrogenase. The resulting two‐domain structure of glycerol‐3‐phos‐phate dehydrogenase allows the correct environment for the B specificity of the nicotinamide ring and theL‐glycerol 3‐phosphate substrate.