The Drosophila NURF remodelling and the ATAC histone acetylase complexes functionally interact and are required for global chromosome organization
The Drosophila NURF remodelling and the ATAC histone acetylase complexes functionally interact and are required for global chromosome organization
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DOI：
10.1038/sj.embor.7401141
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发表时间：
2008-02-01
期刊：
影响因子：
7.7
通讯作者：
Antoniewski, Christophe
中科院分区：
文献类型：
--
作者：
Carre, Clement;Ciurciu, Anita;Antoniewski, Christophe
果蝇 Gcn5 是 SAGA 和 ATAC 组蛋白乙酰化酶复合物的催化亚基。在这里，我们发现 Gcn5 和 ATAC 成分 Ada2a 的突变会诱导男性 X 染色体的解缩，类似于 NURF 核小体重塑复合物的 Iswi 和 Nurf301 亚基突变所诱导的情况。遗传研究以及转录谱分析表明，ATAC 和 NURF 在发育过程中调节重叠的靶基因组。此外，我们发现 Iswi 和 Nurf301 突变体中 Ada2a 染色体结合和组蛋白 H4-Lys12 乙酰化受到损害。我们的结果强烈表明 NURF 是 ATAC 访问染色质和调节全局染色体组织所必需的。
Drosophila Gcn5 is the catalytic subunit of the SAGA and ATAC histone acetylase complexes. Here, we show that mutations in Gcn5 and the ATAC component Ada2a induce a decondensation of the male X chromosome, similar to that induced by mutations in the Iswi and Nurf301 subunits of the NURF nucleosome remodelling complex. Genetic studies as well as transcript profiling analysis indicate that ATAC and NURF regulate overlapping sets of target genes during development. In addition, we find that Ada2a chromosome binding and histone H4-Lys12 acetylation are compromised in Iswi and Nurf301 mutants. Our results strongly suggest that NURF is required for ATAC to access the chromatin and to regulate global chromosome organization.