Embryonic expression pattern of TGF beta type-1 RNA suggests both paracrine and autocrine mechanisms of action.
Embryonic expression pattern of TGF beta type-1 RNA suggests both paracrine and autocrine mechanisms of action.
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发表时间：
1988-10
期刊：
影响因子：
4.6
通讯作者：
S. Lehnert;R. Akhurst
中科院分区：
文献类型：
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作者：
S. Lehnert;R. Akhurst
采用原位杂交技术研究了胎龄为10.5 ~ 15.5天的小鼠胚胎中TGF β 1型RNA的组织分布。正如从成人骨和血小板中TGF β-1蛋白的普遍性所预测的，该RNA在胎儿骨和胎儿肝巨核细胞中高度丰富。我们的数据还揭示了以前未记录的TGF β-1的合成位点，即上皮细胞覆盖那些已知含有TGF β蛋白的间充质组织，如通过免疫组织化学方法（海涅et al. 1987）和某些内脏的间充质组织中所检测到的。结合TGF β多肽（海涅等人，1987）及其mRNA分布的知识，以及已报道的TGF β-1生物活性的知识，我们认为该生长因子的作用机制为旁分泌和自分泌。
The tissue distribution of TGF beta form 1 RNA within mouse embryos of 10.5 to 15.5 days gestational age was investigated using in situ hybridization. As predicted from the prevalence of TGF beta-1 protein in adult bone and platelets, the RNA is highly abundant in fetal bone and in fetal liver megakaryocytes. Our data also reveal previously undocumented sites of synthesis for TGF beta-1, namely epithelia overlying those mesenchymal tissues that are known to contain TGF beta protein as detected by immunohistochemical methods (Heine et al. 1987) and in the mesenchymal tissues of certain internal organs. From a combined knowledge of the distribution of the TGF beta polypeptide (Heine et al. 1987) and its mRNA, and a knowledge of the reported biological activities of TGF beta-1, we invoke both paracrine and autocrine mechanisms of action for this growth factor.